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Method utilizing phage antibody library to screen human histamine receptor 4 (HR4) epitope mimic peptide and vaccine construction method thereof

A technology of epitope mimic peptide and screening method, which is applied in the direction of antibody medical components, peptides, drug combinations, etc., and can solve the problems of unclear liver and kidney effects and high price

Active Publication Date: 2015-11-11
JILIN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

JNJ-7777120 is chemically synthesized, expensive, 3030RMB / 50mg, has oral bioavailability, and the effect on liver and kidney is not yet clear

Method used

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  • Method utilizing phage antibody library to screen human histamine receptor 4 (HR4) epitope mimic peptide and vaccine construction method thereof
  • Method utilizing phage antibody library to screen human histamine receptor 4 (HR4) epitope mimic peptide and vaccine construction method thereof
  • Method utilizing phage antibody library to screen human histamine receptor 4 (HR4) epitope mimic peptide and vaccine construction method thereof

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Embodiment Construction

[0035] In order to make the purpose, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention. Obviously, the described embodiments are part of the present invention Examples, not all examples. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without creative efforts fall within the protection scope of the present invention.

[0036] 1. Main experimental materials

[0037] (1) Target molecule: Anti-HR4antibody (ab178704) monoclonal antibody was purified by Protein A / G in the early stage to obtain a high-purity (>95%) monoclonal antibody that can be used for phage peptide library screening.

[0038] (2) Phage peptide library: Phage random dodecapeptide library (Ph.D-12TMphagedisplaypeptidelibrary) was ...

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Abstract

The invention discloses a method utilizing phage antibody library to screen human histamine receptor 4 (HR4) epitope mimic peptide and a vaccine construction method thereof. A phage random dodecapeptide library is utilized to screen one polypeptide section, and the amino acid sequence of the polypeptide section is FNKWMDCLSVTH. The provided method utilizes a phage random dodecapeptide library technology to screen the polypeptide sequence of HR4 epitope mimic peptide; ELISA identifies the affinity of phage monoclone on HR4 mono-antibody, then sequencing is carried out to obtain one polypeptide sequence, which is names as PT1; the targeting result of phage positive clone is further identified to indicate that phage positive clone can specifically combine the human HR4 monoclonal antibody; the human HR4 monoclonal antibody specific polypeptide can be obtained through screening; thus the HR4 epitope mimic peptide vaccine can be constructed; and the vaccine on the basis of HR4 mimic epitope can be used to treat allergic rhinitis in clinic, can also be applied to the research on HR4 antagonist, and provides primary experimental references for the construction of HR4 antagonist.

Description

technical field [0001] The invention relates to the technical field of medicine and biology, in particular to a method for screening human histamine receptor 4 (HR4) epitope mimic peptides using a phage antibody library and a vaccine construction method thereof. Background technique [0002] Research on HR4 molecules and their antagonists: 1. HR4 molecule histamine receptor-4 (histamine receptor-4, HR4) is a newly discovered histamine receptor, which is a transmembrane G protein-coupled receptor (G- protein-coupled receptors, GPCRs), the function is to inhibit adenylyl cyclase activity, activate phosphoproteinase C (phospholipaseC) and induce calcium ion flow. HR4 is preferentially distributed on immune organs and hematopoietic cells, and is involved in all immune cells of the pathological immune response of allergic rhinitis (AR). The expression of HR4 can be detected in mast cells, CD4+T, CD8+T cells, eosinophils, NK cells, dendritic cells, and monocytes, and has been pro...

Claims

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Application Information

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IPC IPC(8): C07K7/08G01N33/577A61K39/00A61P37/08A61P11/02
Inventor 李琳李东原
Owner JILIN UNIV
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