Method for repolarizing primary hepatocytes in vitro
A primary hepatocyte, repolarization technology, applied in artificial cell constructs, animal cells, vertebrate cells, etc., can solve the problems of insignificant improvement and late appearance of repolarization, achieving compact arrangement, The effect of enhancing cell function in vitro
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[0041] Embodiment 1, microfluidic perfusion culture method
[0042] This example provides a method for in vitro repolarization of primary hepatocytes, comprising the following steps: 50 μL of 6 The cell / mL primary hepatocyte suspension flowed through at a flow rate of 0.02 mL / h by means of circulatory perfusion and naturally retained in the cell culture pool. At the same time, under an ordinary inverted optical microscope, a simple compact force is applied to the primary hepatocyte population by gently wriggling with ophthalmic tweezers, so that the cell density per unit volume reaches more than 90%. Cells were cultured at 37°C, 5% CO in serum-free medium for hepatocyte phenotype after planting. 2 perfusion culture conditions. In the first hour, hepatocyte phenotype serum-free culture medium was used for perfusion culture at a flow rate of 0.02mL / h, and in the second to 4h, hepatocyte phenotype serum-free culture medium was used for culture at a low flow rate (0.02-0.03mL / h)...
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