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Rolling circle amplification-terahertz metamaterial biosensor and method for rapid detection of multidrug-resistant Mycobacterium tuberculosis

A biosensor, Mycobacterium tuberculosis technology, applied in the field of detection, can solve the problems of poor stability of results and long cycle, and achieve the effect of stable and reliable results

Active Publication Date: 2017-11-24
THE FIRST AFFILIATED HOSPITAL OF THIRD MILITARY MEDICAL UNIVERSITY OF PLA
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  • Abstract
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Problems solved by technology

However, at present, RCA is mainly detected by agarose gel electrophoresis, which has a long cycle and poor stability of results.

Method used

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  • Rolling circle amplification-terahertz metamaterial biosensor and method for rapid detection of multidrug-resistant Mycobacterium tuberculosis
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  • Rolling circle amplification-terahertz metamaterial biosensor and method for rapid detection of multidrug-resistant Mycobacterium tuberculosis

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Embodiment Construction

[0030] The preferred embodiments of the present invention will be described in detail below with reference to the accompanying drawings. For the experimental methods not specified in the examples, the conventional conditions are generally followed, such as the conditions described in the Molecular Cloning Experiment Guide (Third Edition, J. Sambrook et al.), or the conditions suggested by the manufacturer.

[0031]Rolling circle amplification-terahertz metamaterial biosensor, comprising a terahertz metamaterial, a capture probe coated in a reaction region of the terahertz metamaterial to identify a template sequence to be detected, and a polystyrene slide, the polystyrene slide Covering the upper layer of the terahertz metamaterial and forming a liquid film cavity for liquid phase amplification reaction, the height of the liquid film cavity is about 50 μm; the structure of the terahertz metamaterial is as follows figure 1 As shown, it is composed of metal split resonators peri...

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Abstract

The invention relates to a rolling circle amplification-terahertz metamaterial biosensor and a method for rapidly detecting multi-drug-resistant Mycobacterium tuberculosis. The biosensor includes a terahertz metamaterial and is coated in a terahertz metamaterial reaction zone to identify a template sequence to be detected. The capture probe and the polystyrene glass slide are covered on the upper layer of the terahertz metamaterial and form a liquid film cavity for the liquid phase amplification reaction, and the rolling circle amplification-terahertz metamaterial is The capture probe coated by the biosensor hybridizes with the circular template of rolling circle amplification to achieve in-situ replication amplification. The results of using the method of the present invention are stable and reliable, and five drug resistance levels of multi-drug-resistant Mycobacterium tuberculosis can be completed within 3 hours. Detection of sites.

Description

technical field [0001] The invention belongs to the detection field, and in particular relates to a rolling circle amplification-terahertz metamaterial biosensor, and also relates to a method for rapidly detecting multidrug-resistant Mycobacterium tuberculosis by the rolling circle amplification-terahertz metamaterial biosensor. Background technique [0002] In recent years, various factors such as the increase of population mobility have made the infection rate of Mycobacterium tuberculosis remain high, and tuberculosis is spreading rapidly to the world. The incidence of multi-drug resistant Mycobacterium tuberculosis is increasing due to delay in diagnosis of the disease and overuse of anti-tuberculosis drugs. Ordinary tuberculosis only needs to take first-line anti-tuberculosis drugs (such as: isoniazid, rifampicin, etc.), and adopt the principles of early, joint, appropriate, regular and full course, and it can be cured in about half a year. Mycobacterium tuberculosis i...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M1/34C12Q1/68C12Q1/04C12R1/01
CPCC12Q1/682C12Q1/6825C12Q2531/125C12Q2543/101C12Q2537/162C12Q2565/607G01N21/3586
Inventor 杨翔府伟灵黄庆罗阳刘跃平余抒徐含青赵祥刘羽杨柯黄娇祺
Owner THE FIRST AFFILIATED HOSPITAL OF THIRD MILITARY MEDICAL UNIVERSITY OF PLA
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