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A kind of tissue culture method of Fritillaria sichuan

A technique of tissue culture and fritillary, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problem of small number and so on

Active Publication Date: 2018-03-02
云南昊辰农业有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In the existing patents, the tissue culture of Fritillaria has been reported, but the number is small, and the varieties involved are Dark Purple Fritillaria, Hubei Fritillaria, Yili Fritillaria, Flat Fritillaria, Yellow Flower Fritillaria, Anhui Fritillaria, Gansu Fritillaria Fritillaria etc., among them, the report about Fritillaria cirrhosaD.Don is less, Wang Yuehua etc. carried out the induction research of callus with the root of Fritillaria cirrhosaD.Don as explant

Method used

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  • A kind of tissue culture method of Fritillaria sichuan
  • A kind of tissue culture method of Fritillaria sichuan
  • A kind of tissue culture method of Fritillaria sichuan

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Embodiment 1

[0026] (1) Selection and treatment of explants: choose fresh and healthy bulbs of Fritillaria sichuanensis as explants, clean the soil with a soft brush under tap water, remove the fibrous roots of the bulbs, and then dilute with a small amount of detergent solution (1g dissolved in 100ml distilled water) to clean the surface of the explant, rinse it with tap water, and rinse it for about 30 minutes; for the bulbs whose scales and bulbs are more tightly healed, clean the entire surface of the bulb, if the scales of the bulb and the bulb There is a gap between the hearts, in order to wash the dirt inside, the scales and the scales need to be separated and cleaned, and washed for later use;

[0027] (2) Disinfection of explants: use the bulbs of Fritillaria sichuanensis as explants, wash the bulbs of Fritillaria chinensis with tap water, wash the soil, remove the fibrous roots, soak them in 75% ethanol for 10 seconds on the ultra-clean workbench, and wash them with sterile water ...

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Abstract

The invention discloses a tissue cultivation method of bulbus fritillariae cirrhosae. Fritillaria cirrhosa D.Don is a Liliaceae Fritillaria perennial medicinal herb, underground bulbs are used as medicine, the bulbus fritillariae cirrhosae has the effects of clearing away heat and moistening the lungs, preventing phlegm from forming and stopping coughing, and is one of the main sources for the famous traditional Chinese medicinal material bulbus fritilariae specified in the Chinese Pharmacopoeia (Year 2005). The bulbus fritillariae cirrhosae is distributed in high altitude localities, is propagated through seeds, and is low in reproductive rate and long in growth cycle; due to long-term harvesting and wild resource deficiency, the problem of large market demand needs to be solved urgently. Bulbs of the bulbus fritillariae cirrhosae are used as explants, bulbus fritillariae cirrhosae in-vitro regenerated plants are obtained through the processes of explant sterilization, induction of callus tissues in two different states, bulb differentiation, multiplication, rooting culture, acclimatization and transplantation and the like, an effective tissue culture rapid propagation system is established, a large number of seedlings can be produced in a short period of time, the breeding efficiency is improved, and the propagation and popularization of the bulbus fritillariae cirrhosae are facilitated.

Description

technical field [0001] The invention belongs to the field of biotechnology, in particular to a method for tissue culture of Fritillaria cirrhosa D.Don. Background technique [0002] Fritillaria cirrhosa D.Don is a perennial herbal medicinal plant of the genus Fritillaria genus in the family Liliaceae. The medicinal part is the underground bulb. It has the effects of clearing away heat and nourishing the lungs, reducing phlegm and relieving cough. It is an important medicine for treating cough. Chuan Fritillaria, Dark Purple Fritillaria (Fritillaria unibracteata Hsiao et K.C.Hsia), Gansu Fritillaria (Fritillariaprzewalskii Maxim), Suosha Fritillaria (Fritillaria delavayi Franch.) are well-known traditional Chinese medicines stipulated in "Chinese Pharmacopoeia" (2005) The main source of Chuanbei. Chuan Fritillaria is mainly produced in Sichuan, Tibet, Yunnan and other provinces and regions. It likes cold and cool climate conditions. It has the characteristics of cold resista...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 尹进超邓清张根松杨晨璇熊毅
Owner 云南昊辰农业有限公司
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