Potato virus-free test-tube seedling direct transplanting method

A test-tube seedling and potato technology, which is applied in the directions of botanical equipment and methods, cultivation, disinfectants, etc., can solve the problem of low transplant survival rate of potato detoxification test-tube seedlings, large mechanical damage of potato detoxification test-tube seedlings, and potato detoxification test-tube seedlings. The problem of increasing the pollution rate of seedlings, etc., can achieve the effect of reducing the links of seedling refining and washing, reducing the links of washing seedlings, and reducing the links of seedling curing.

Inactive Publication Date: 2016-07-06
丽江市农业科学研究所
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Problems solved by technology

The main problems of this method are: the one, the contamination rate of potato detoxified test tube seedlings increases in th

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Embodiment Construction

[0007] Potato virus-free test-tube seedlings were cultured in the tissue culture room at a temperature of 22-24°C and a light of 2200-2500Lx for 9-11 days, and then continued to grow in the tissue culture room for 5-5 days at a temperature of 16-18°C and light of 2800-3000Lx. 8 days, and then transplanted into the greenhouse, before transplanting to the seedbed substrate, use carbendazim 0.23~0.30g / ㎡, silver fare 0.09~0.11ml / ㎡, Dasheng 0.18~0.27g / ㎡ and BEST 0.07~0.11 After mixing in ml / ㎡, add water 45-60ml / ㎡ to dilute, and spray for disinfection.

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Abstract

The invention provides a potato virus-free test-tube seedling direct transplanting method.The method is characterized in that after a potato virus-free test-tube seedling is cultured for 9-11 days in a tissue culture room under the conditions that the temperature is 22-24 DEG C and illumination intensity is 2200-2500 Lx, the potato virus-free test-tube seedling continues to be cultured for 5-8 days in the tissue culture room under the conditions that the temperature is 16-18 DEG C and illumination intensity is 2800-3000 Lx, the potato virus-free test-tube seedling is transplanted into a greenhouse, sprayed and sterilized with a mixture obtained after carbendazim with the concentration being 0.23-0.30g/m<2>, Yinfali with the concentration being 0.09-0.11 mg/m<2>, mancozeb with the concentration being 0.18-0.27 g/m<2> and alpha-cypermethrin with the concentration being 0.07-0.11 ml/m<2> are mixed and diluted by adding water with the concentration being 45-60 ml/m<2> before being transplanted to a seedbed medium.By means of potato virus-free test-tube seedling transplanting, the survival rate is high, the seedling hardening step and the seedling washing step are reduced, the production period is shortened, labor investment is reduced, and production cost of mother seeds of potatoes is reduced.

Description

technical field [0001] The invention relates to a method for direct transplanting of virus-free potato seedlings in test tubes. Background technique [0002] The yield and quality of potatoes are closely related to the quality of potato seeds. If the virus is contained in the seed potato, the quality of the potato will be seriously degraded, and various diseases will occur, resulting in a sharp drop in potato yield. Using shoot tip tissue culture technology is the main way to produce virus-free potato seeds. The process of producing the original potato seed by the traditional matrix cultivation method is to cultivate the potato virus-free test tube seedlings in the tissue culture room for 15-18 days, then place them in a greenhouse or a place with low light to harden the seedlings for about 7 days, then wash the seedlings and transplant them into the greenhouse . The main problems of this method are: the one, the contamination rate of potato detoxified test tube seedlings...

Claims

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Application Information

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IPC IPC(8): A01G1/00A01G31/00A01N47/18A01N43/40A01N47/12A01P1/00A01P3/00
CPCA01G31/00A01N43/40A01N47/12A01N47/18A01G22/00A01N2300/00
Inventor 和习琼和光宇王菊英王绍林和平根和忠和生鼎杨群擎和国钧张凤文高政
Owner 丽江市农业科学研究所
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