Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A method for biosynthesizing nano-silver through the extract of star anise gold disc leaves

An octagonal gold plate, biosynthesis technology, applied in the biological field, can solve the problems of limited sources of plant materials, and achieve the effect of low price, fast synthesis speed, and high antibacterial activity

Active Publication Date: 2021-09-03
HUAZHONG AGRI UNIV
View PDF6 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to resource and geographical constraints, the sources of different plant materials are limited and may only meet the production needs of specific regions

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A method for biosynthesizing nano-silver through the extract of star anise gold disc leaves
  • A method for biosynthesizing nano-silver through the extract of star anise gold disc leaves
  • A method for biosynthesizing nano-silver through the extract of star anise gold disc leaves

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1 Biosynthesis of nano-silver particles by star anise gold plate leaf extract

[0031] a. Preparation of biological matrix of star anise gold plate leaf extract

[0032] The extracted leaves of Antonia chinensis were washed with deionized water, dried in an oven at 65°C, and then dried by a pulverizer to make powder and collected for future use.

[0033] Weigh 1 g of the star anise gold disc powder, add 35 mL of deionized water, shake at 37°C, 180 rpm for 10 min; centrifuge at 12000 rpm for 20 min, collect the supernatant, and freeze-dry to obtain the star anise gold disc leaf extract, which is used as a matrix for nano-silver synthesis.

[0034] b. Synthesis of nano silver

[0035] Add 1 mM AgNO to the synthetic matrix at concentrations of 0, 2, 4, 6, 8 mg / mL 3 The solution was reacted for 80min under the irradiation of LED lamp (42000lx).

[0036] c. Characterization of nano silver

[0037] The synthesis of nano-silver can be characterized by color change ...

Embodiment 2

[0038] Example 2 Biosynthesis of nano-silver particles by the leaf extract of star anise gold disc

[0039] a. Preparation of biological matrix

[0040] The extracted leaves of Antonia chinensis were washed with deionized water, dried in an oven at 65°C, and then dried by a pulverizer to make powder and collected for future use.

[0041] Weigh 1 g of the star anise gold disc powder, add 35 mL of deionized water, shake at 37°C, 180 rpm for 10 min; centrifuge at 12000 rpm for 20 min, collect the supernatant, and freeze-dry to obtain the star anise gold disc leaf extract, which is used as a matrix for nano-silver synthesis.

[0042] b. Synthesis of nano silver

[0043] Add 0.5, 1, 2, 3 mM AgNO to the synthetic matrix at a concentration of 8 mg / mL 3 , under the condition of LED lamp irradiation (42000lx), react for 80min.

[0044] c. Characterization of nano silver

[0045] After adding different concentrations of AgNO 3 Under the condition of figure 2 ), indicating the add...

Embodiment 3

[0046] Example 3 Biosynthesis of nano-silver particles by star anise gold plate leaf extract

[0047] a. Preparation of biological matrix

[0048] Wash the extracted leaves of Antonia chinensis with deionized water, dry them in an oven at 65°C, and then use a pulverizer to make the dried leaves of Antonia chinensis into powder, and collect them for later use.

[0049] Weigh 1 g of the star anise gold plate powder, add 35 mL of deionized water, shake at 37 ° C, 180 rpm for 10 min; centrifuge at 12000 rpm for 20 min, collect the supernatant, and freeze-dry to obtain the star anise gold plate leaf extract, which is used as a matrix for nano-silver synthesis.

[0050] b. Synthesis of nano silver

[0051] Add 2mM silver nitrate solution to the synthetic matrix with a concentration of 8mg / mL, and react for 80min under the conditions of LED light irradiation (7000, 11000, 20000, 42000lx).

[0052] c. Characterization of nano silver

[0053] Such as image 3 It shows that nano-silve...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
particle diameteraaaaaaaaaa
Login to View More

Abstract

The invention relates to the field of biotechnology, in particular, the invention relates to a method for biosynthesizing nano-silver through extracts from leaves of star anise aurantia discus. The method comprises the following steps: 1) washing the leaves of the star anise gold plate for 3 times, drying at 60°C, grinding them into powder, adding ultrapure water at 180r / min to shake for 10 minutes, centrifuging at 12000rpm for 20 minutes, and freeze-drying the supernatant to obtain star anise gold Disc leaf extract; 2) take the star anise disc leaf extract in step 1) as reaction matrix, add AgNO 3 solution, so that the concentration of the star anise leaf extract in the resulting mixed solution is 8-10mg / mL, AgNO 3 The concentration is 0.5‑3mM, and the reaction is performed under the irradiation condition of LED light to obtain nano-silver particles. The octagonal gold disc leaf material used in the biosynthesis method of the present invention has a wide range of sources, is safe and environmentally friendly, and has a high synthesis speed, and can complete the reaction within 80-100 minutes, and is a green, efficient and safe biosynthesis method.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular, the invention relates to a method for biosynthesizing nano-silver through extracts from leaves of star anise aurantia discus. Background technique [0002] Nanomaterials are a research hotspot in recent years. Quantum mechanical effects at the nanoscale endow nanomaterials with various excellent properties. Nanosilver, that is, silver atoms or clusters of silver atoms with a particle size of 1-100nm, has a variety of excellent physical, chemical and optical properties, such as antibacterial properties and surface-enhanced Raman scattering effects. Nanosilver has good application potential in the fields of antibacterial materials, molecular detection and catalysts, and has attracted widespread attention of scientific researchers in recent years (see reference 1 for details: Chaloupka K, Malam Y, Seifalian A M. Trends Biotechnol., 2010, 28 :580-588). [0003] The traditional nano-silve...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12P3/00
CPCC12P3/00
Inventor 魏雪团司果果张璟邹俊范瑞梁
Owner HUAZHONG AGRI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com