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Kit for testing clozapine application effect based on rs2032582 and application method of kit

A kit, clozapine technology, applied in the field of genetics, can solve problems such as undiscovered

Inactive Publication Date: 2016-08-17
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

According to the search of the existing literature, the applicant did not find any related reports that the No. 8 exon SNPrs2032582 of the ABCB1 gene of the present invention is a molecular marker of clozapine

Method used

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  • Kit for testing clozapine application effect based on rs2032582 and application method of kit
  • Kit for testing clozapine application effect based on rs2032582 and application method of kit
  • Kit for testing clozapine application effect based on rs2032582 and application method of kit

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Embodiment

[0036] The invention relates to a method for detecting the single nucleotide polymorphism of the eighth exon SNP rs2032582 of the ABCB1 gene, as well as related isolated nucleic acids and primers. Through association analysis, the present invention unexpectedly discovered that SNP rs2032582 on the eighth exon of the ABCB1 gene can be used as a molecular marker for clozapine. The specific information of the ABCB1 gene is ABCB1 gene LOCUS NC_000007, with a full length of 209461bp. The present invention uses Taqman- The MGB probe technology detected the SNP site of the eighth exon of the ABCB1 gene and its frequency distribution, and found that the C→T polymorphism at position 27439 of the eighth exon of the ABCB1 gene, that is, the encoded protein The 893rd amino acid Arg(R)→His(H) polymorphism in the sequence is SNP rs2032582. The ABCB1 gene is at the chromosome position: Homo sapiens chromosome7, GRCh38.p2 (87503863..87713323), and the SNP rs2032582 is at the chromosome positi...

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Abstract

The invention relates to a kit for testing clozapine application effect based on rs2032582 and an application method of the kit, and particularly relates to a method for testing single nucleotide polymorphism of ABCB1 (ATP binding cassette subfamily B member 1) NO.8 exon in the field of genes. Sequence of the nucleotide, which is isolated and comprises single nucleotide polymorphism locus, is as shown as SEQ ID NO:1; the single nucleotide polymorphism locus is located at NO.3169, rs2032582 (C->T). The method for testing the nucleotide includes the steps of firstly, testing the nucleotide at the locus NO.3169 of the sequence as shown as SEQ ID NO:1; secondly, testing whether there is of the single nucleotide polymorphism at the locus NO.3169 or not by means of a Taqman-MGB typing method. According to the kit, the application method thereof and the method for testing the nucleotide, a foundation for research on ABCB1 gene polymorphism and clinical medicine safety is laid, a theoretical basis for individualization of clinical medicine is provided, and a guidance for research and development of novel medicines based on pharmacogenomics idea is also provided.

Description

technical field [0001] The invention relates to the field of gene technology, in particular to a method for detecting the single nucleotide polymorphism of the eighth exon of the ABCB1 gene. Background technique [0002] The chemical name of Clozapine is 8-chloro-11-(4-methyl-1-piperazinyl)-5H-dibenzo[b,e][1,4]diazepine, molecular formula C18H19ClN4 . Clozapine is the most common drug for the treatment of mental illness in China. It is not only effective for positive symptoms of psychosis, but also for negative symptoms. It is suitable for all subtypes of acute and chronic schizophrenia, and has a good effect on hallucination, delusional and youthful schizophrenia. It can also reduce the affective symptoms (such as depression, guilt, anxiety, etc.) associated with schizophrenia. However, there are obvious individual differences in the efficacy and side effects of clozapine in clinical practice, because its metabolism is affected by genetic factors and environmental factor...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6883C12Q2600/106C12Q2600/156
Inventor 秦胜营周伟徐青青沈陆贺林
Owner SHANGHAI JIAO TONG UNIV
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