Test method of reaction of EPHX1 genotype based COPD to NAC
A test method and genotype technology, applied in the field of testing the response of EPHX1 genotype COPD to NAC, can solve the problem of not being able to show the different curative effects of N-acetylcysteine antioxidant therapy in COPD patients
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[0020] In order to make the objectives, technical solutions and advantages of the present invention clearer, the present invention will be further described in detail below with reference to the embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention, but not to limit the present invention.
[0021] The application principle of the present invention will be described in detail below with reference to the accompanying drawings.
[0022] like figure 1 As shown, the test method based on the EPHX1 genotype COPD response to NAC according to the embodiment of the present invention comprises the following steps:
[0023] S101: DNA is extracted by phenol / chloroform method, followed by polymerase chain reaction PCR amplification;
[0024] S102: Polyacrylamide electrophoresis, laser scanning automatic DNA sequencing, mEPH gene activity is divided into four types according to different gene polymorphisms of EPHX...
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