Stop-flow two-dimensional liquid chromatography with two multi-port valves as switching devices and its application

A two-dimensional liquid chromatography and liquid chromatography technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of instantaneous pressure changes and pressure mutations when the flow stops, and achieve stable pressure, extended life, and convenient operability Effect

Active Publication Date: 2017-12-01
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In order to overcome the problem that the existing stop-flow liquid chromatography flow channel has a large pressure change at the moment of stop flow, the primary purpose of the present invention is to provide a stop-flow two-dimensional liquid chromatography, which is composed of two multi-port valves The flow switching device successfully solves the problem of sudden pressure change at the moment of stop-flow switching, and successfully realizes the split flow of the first-dimensional chromatography, which improves the detection efficiency of stop-flow two-dimensional liquid chromatography

Method used

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  • Stop-flow two-dimensional liquid chromatography with two multi-port valves as switching devices and its application
  • Stop-flow two-dimensional liquid chromatography with two multi-port valves as switching devices and its application
  • Stop-flow two-dimensional liquid chromatography with two multi-port valves as switching devices and its application

Examples

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Embodiment 1

[0046] A stopped-flow two-dimensional liquid chromatography, such as Figure 1-2 As shown, it includes a first-dimensional separation part, a stop-flow switching device and a second-dimensional separation detection part;

[0047] The stop-flow switching device is composed of two six-way valves, wherein the six-way valve f for stop-flow is connected to the first-dimensional separation part, and the six-way valve g for switching is connected to the second-dimensional separation detection part. All are three-slot two-position six-way valves (ValcoEUDB-C6W);

[0048] When in the sampling state ( figure 1 ), the first-dimension liquid chromatography pump a pumps the mobile phase into the sampling device b, and pumps the samples together to the first-dimension liquid chromatography column c for separation, and the effluent components pass through the three-way pipe d and are divided into two flow paths , assuming that the lengths of the two flow paths after the tee pipe are equal,...

Embodiment 2

[0054] A stopped-flow two-dimensional liquid chromatography, such as Figure 3-4 As shown, the difference from Example 1 is that the multi-way valve f for stopping flow is a two-slot, two-position, four-way valve, and the multi-way valve g for switching is a four-slot, two-position, eight-way valve, and the multi-way valve for switching is a two-position, four-way valve. The 7th and 8th positions are connected by a chromatographic tube. In addition, the splitting device d adopts a four-way pipe, and the first-dimensional liquid chromatography pump a adopts a double pump. Other than that, its structure and working principle are consistent with Embodiment 1.

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Abstract

The invention discloses a flow-stopping type two-dimensional liquid chromatogram adopting two multi-way valves as switching devices and an application thereof. The two-dimensional liquid chromatogram comprises a first-dimensional separating part, a flow-stopping switching device and a second-dimensional separating and detecting part, wherein the flow-stopping switching device consists of two multi-way valves; the multi-way valve for stopping flow is connected with the first-dimensional separating part, and the multi-way valve for switching is connected with the second-dimensional separating and detecting part. The flow-stopping type two-dimensional liquid chromatogram disclosed by the invention has the advantages that an outflow component of the first-dimensional liquid chromatogram is connected to two adjacent input ends of the multi-way valve, and when the sampling state is ended, the valves are rotated simultaneously at the moment when the flowing speed of a first-dimensional liquid chromatographic pump is stopped, and the pressure of a first-dimensional liquid chromatographic column can be maintained, so that the problem of large pressure change when the flow is stopped is solved, and the capabilities of separation and analysis are greatly improved.

Description

technical field [0001] The invention belongs to the field of instrument analysis, in particular to a stop-flow two-dimensional liquid chromatography, in particular to a stop-flow two-dimensional liquid chromatography with two multi-way valves as switching devices and its application. Background technique [0002] Two-dimensional liquid chromatography is a high-efficiency two-dimensional separation and analysis technology developed on the basis of one-dimensional high-performance liquid chromatography. All or part of it is transferred to the second-dimensional high-performance liquid chromatography system to achieve efficient separation and analysis of complex components. [0003] Since valve switching technology plays a vital role in the connection between two dimensions and the transfer of components, it has always been regarded as the core technology of multidimensional liquid chromatography. The multi-way valve is mainly composed of a circular gasket (rotor) and a fixed ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/46
CPCG01N30/02G01N30/468G01N2030/027
Inventor 赵谋明徐巨才苏国万赵容钟
Owner SOUTH CHINA UNIV OF TECH
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