Stop-flow two-dimensional liquid chromatography with two multi-port valves as switching devices and its application
A two-dimensional liquid chromatography and liquid chromatography technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of instantaneous pressure changes and pressure mutations when the flow stops, and achieve stable pressure, extended life, and convenient operability Effect
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Embodiment 1
[0046] A stopped-flow two-dimensional liquid chromatography, such as Figure 1-2 As shown, it includes a first-dimensional separation part, a stop-flow switching device and a second-dimensional separation detection part;
[0047] The stop-flow switching device is composed of two six-way valves, wherein the six-way valve f for stop-flow is connected to the first-dimensional separation part, and the six-way valve g for switching is connected to the second-dimensional separation detection part. All are three-slot two-position six-way valves (ValcoEUDB-C6W);
[0048] When in the sampling state ( figure 1 ), the first-dimension liquid chromatography pump a pumps the mobile phase into the sampling device b, and pumps the samples together to the first-dimension liquid chromatography column c for separation, and the effluent components pass through the three-way pipe d and are divided into two flow paths , assuming that the lengths of the two flow paths after the tee pipe are equal,...
Embodiment 2
[0054] A stopped-flow two-dimensional liquid chromatography, such as Figure 3-4 As shown, the difference from Example 1 is that the multi-way valve f for stopping flow is a two-slot, two-position, four-way valve, and the multi-way valve g for switching is a four-slot, two-position, eight-way valve, and the multi-way valve for switching is a two-position, four-way valve. The 7th and 8th positions are connected by a chromatographic tube. In addition, the splitting device d adopts a four-way pipe, and the first-dimensional liquid chromatography pump a adopts a double pump. Other than that, its structure and working principle are consistent with Embodiment 1.
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