Stop-flow two-dimensional liquid chromatography with a multi-port valve as switching device and its application

A technology of two-dimensional liquid chromatography and switching devices, which is applied in the direction of measuring devices, solid adsorbent liquid separation, instruments, etc. It can solve the problems of large pressure changes at the moment of stop flow, sudden pressure changes, and inability to split flow, etc., to reduce pressure pulses , improve accuracy, reduce the effect of steps and links

Active Publication Date: 2018-04-27
SOUTH CHINA UNIV OF TECH
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  • Summary
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  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] In order to overcome the problem that the existing stop-flow liquid chromatography flow path has a large pressure change at the moment of stop flow and cannot split the flow, the primary purpose of the present invention is to provide a stop-flow two-dimensional liquid chromatography, which adopts a multi-pass The valve is a flow switching device, which successfully solves the problem of sudden pressure changes at the moment of stop-flow switching, and successfully realizes the split flow of the first-dimensional chromatography, which improves the detection efficiency of stop-flow two-dimensional liquid chromatography

Method used

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  • Stop-flow two-dimensional liquid chromatography with a multi-port valve as switching device and its application
  • Stop-flow two-dimensional liquid chromatography with a multi-port valve as switching device and its application
  • Stop-flow two-dimensional liquid chromatography with a multi-port valve as switching device and its application

Examples

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Embodiment 1

[0043] A stopped-flow two-dimensional liquid chromatography, such as Figure 1-2 As shown, it includes a first-dimensional separation part, a stop-flow switching device, and a second-dimensional separation detection part;

[0044] The stop-flow switching device is a four-slot two-position eight-way valve e;

[0045] When in the sampling state ( figure 1 ), the first-dimensional liquid chromatography pump a pumps the mobile phase to the autosampler b, and pumps the sample into the chromatographic column c. The effluent components are then split through the three-way pipe d, and one of the flow paths passes through the multi-way valve Positions 2 and 1 of e flow to the waste tank through the pressure balance loop g, and the other flow path passes through positions 3 and 4 of the multi-way valve e in turn, and then enters the sampling loop f for filling, and then passes Positions 7 and 8 flow to the waste liquid bucket, and the effluent of the second-dimensional liquid chromatography ...

Embodiment 2

[0053] A stopped-flow two-dimensional liquid chromatography, such as Figure 3-4 As shown, the difference from Embodiment 1 is that the multi-way valve e for stopping flow is a five-slot, two-position, ten-way valve, and positions 9 and 10 are closed with plugs. In addition, the sampling device b adopts a manual injector, and the second-dimensional liquid chromatography pump h adopts a double pump. Otherwise, its structure and working principle are the same as the first embodiment.

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Abstract

The invention discloses a stop-flow two-dimensional liquid chromatograph adopting a multi-ported valve as a switching device, and an application thereof. The stop-flow two-dimensional liquid chromatograph comprises a first-dimensional separation part, a stop-flow switching device and a second-dimensional separation detection part, and the stop-flow switching device is a multi-ported valve. The stop-flow two-dimensional liquid chromatograph allows the efflux component of the first-dimensional liquid chromatogram to be connected to two adjacent input ends of the multi-ported valve and the multi-ported valve to be rotated at the stop-flow moment, so sealing of the flow path of a first-dimensional liquid chromatogram system is realized, thereby the pressure of the first-dimensional liquid phase chromatogram is kept, the pressure pulse of the first-dimensional liquid chromatogram system is reduced, and the problem of large pressure change at the stop-flow moment is solved; and control of the sample introduction and the elution of second-dimensional liquid chromatogram is realized, steps and links for intermediate control are reduced, and the control accuracy is substantially improved.

Description

Technical field [0001] The invention belongs to the field of instrument analysis, and specifically relates to a stopped-flow two-dimensional liquid chromatography, in particular to a stopped-flow two-dimensional liquid chromatography with a multi-way valve as a switching device and its application. Background technique [0002] As a new type of liquid chromatography separation technology, two-dimensional liquid chromatography has a resolution far superior to that of one-dimensional liquid chromatography, so it is favored by scholars at home and abroad, and it is a hot spot in current chromatographic separation research. [0003] Because the multi-port valve plays a vital role in the connection between two dimensions and the transfer of components, it has always been regarded as a key part of multi-dimensional liquid chromatography. The multi-port valve is mainly composed of a circular gasket (rotor) and a fixed base (stator). There are a certain number of slots on the rotor to guid...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01D15/22B01D15/14B01D15/24G01N30/02
CPCB01D15/14B01D15/22B01D15/24G01N30/02G01N2030/027
Inventor 苏国万赵谋明徐巨才赵强忠
Owner SOUTH CHINA UNIV OF TECH
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