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System and method for presenting large DNA molecules for analysis

A nucleic acid molecule, ionic strength technology, applied in chemical instruments and methods, analytical materials, material analysis by electromagnetic means, etc., can solve problems such as limitations, increasing the complexity of molecular load processing, and demanding requirements.

Active Publication Date: 2016-09-07
WISCONSIN ALUMNI RES FOUND +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Both techniques show limitations, although with stretches above 0.8
The method of Reisner et al. is more demanding, which requires the fabrication of extremely nanoconfined devices smaller than the molecular persistence length. 16 , to elongate the DNA molecule close to the molecular straight length, thus, this increases the complexity of the molecular loading process

Method used

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  • System and method for presenting large DNA molecules for analysis
  • System and method for presenting large DNA molecules for analysis
  • System and method for presenting large DNA molecules for analysis

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Embodiment Construction

[0020] All referenced patents, applications, and non-patent literature cited in this disclosure are hereby incorporated by reference in their entirety. In the event of a discrepancy between a reference and the present disclosure, the present disclosure controls.

[0021] The present disclosure provides microfluidic devices. The microfluidic device can include a first microchannel, a second microchannel, a nanoslit, nucleic acid molecules, and an ionic buffer. A nanoslit can extend between the first and second microchannels. The nanoslit can provide a fluid path between the first and second microchannels. A nucleic acid molecule can have a first end, a second end, and a middle portion between the first end and the second end. Ionic buffers can be in the nanoslits and the first and second microchannels. The first microchannel may include a first clustered region near the first end of the nanoslit, and the second microchannel may include a second clustered region near the sec...

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Abstract

Systems and methods for presenting nucleic acid molecules for analysis are provided. The nucleic acid molecules have a central portion that is contained within a nanoslit. The nanoslit contains an ionic buffer. The nucleic acid molecule has a contour length that is greater than a nanoslit length of the nanoslit. An ionic strength of the ionic buffer and electrostatic or hydrodynamic properties of the nanoslit and the nucleic acid molecule combining to provide a summed Debye length that is greater than or equal to the smallest physical dimension of the nanoslit.

Description

[0001] Cross References to Related Applications [0002] This application claims priority to US Provisional Patent Application Serial No. 61 / 877,570, filed September 13, 2013, the disclosure of which is incorporated herein by reference in its entirety. [0003] Statement Regarding Federally Funded Research / Development [0004] This invention was made with government support under grant HG000225 awarded by the National Institutes of Health and 0832760 awarded by the National Science Foundation. The government has certain rights in this invention. Background of the invention [0005] The present invention relates to the field of nucleic acid molecule manipulation. More specifically, the invention relates to stretching nucleic acid molecules to better visualize certain portions of the nucleic acid molecule for observation by various techniques. [0006] A large portion of the human genome contains DNA sequences that exist in multiple copies. Although such elements play import...

Claims

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Application Information

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IPC IPC(8): B01L3/00G01N33/487
CPCB01L3/502761G01N33/48721B01L2200/0663B01L2300/0816B01L2300/0896B01L7/52G01N27/44791B01L2300/18
Inventor D·C·施瓦茨K·考纳福斯基-谢弗J·埃尔南德斯-奥尔蒂斯T·奥代克J·迪帕波罗K·朱
Owner WISCONSIN ALUMNI RES FOUND
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