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A biological product stabilizer containing fish gelatin

A technology of biological products and fish gelatin, applied in biological testing, material inspection products, measuring devices, etc., can solve the problems of high price, limited scope of application, cumbersome preparation process, etc., and achieve low price, wide application scope and simple formula Effect

Active Publication Date: 2018-02-06
WUHAN CLOUD CLONE CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The technical problem to be solved by the present invention is that some existing conventional protein, antigen, antibody, biological detection and diagnostic kits and other biological product stabilizers are expensive, the preparation process is cumbersome, and the scope of application is limited. A new fish-containing gelatin is provided stabilizers for biological products

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  • A biological product stabilizer containing fish gelatin
  • A biological product stabilizer containing fish gelatin
  • A biological product stabilizer containing fish gelatin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Fish gelatin 15-25g / L, Tris 4.526-5.045g / L, sodium edetate 14-22g / L, trehalose 25-30g / L, Tween-80 3-6g / L , Biological preservative Proclin 300 0.5 ~ 2.0g / L, use double-distilled water to prepare a solution in proportion, filter and sterilize to obtain a stabilizer, and store at 4 degrees. BCA protein concentration determination kit (purchased from Biyuntian, product number: P0011). Microplate reader (ELx808 Biotek).

[0025] Protein freezing solution A: conventional protein freezing solution (40% glycerol aqueous solution, v / v);

[0026] Protein cryopreservation solution B: add 5% stabilizer (v / v) to conventional protein cryopreservation solution.

[0027] Randomly select 6 protein numbers 1, 2, 3, 4, 5, and 6 from the protein library, divide the samples into 20 tubes at 50 μg / ml for each protein, take half of each protein, and add protein freezing solution A and B. After storage at -20°C for a period of time, the protein concentration was determined according to the...

Embodiment 2

[0034] Fish gelatin 25g / L, trishydroxymethylaminomethane 5.045g / L, sodium edetate 20g / L, trehalose 25g / L, Tween-80 3-6g / L, biological preservative Proclin 300 0.5- 2.0g / L, use double-distilled water to prepare a solution in proportion, filter and sterilize to obtain a stabilizer. BCA protein concentration determination kit (purchased from Biyuntian, product number: P0011). Microplate reader (ELx808 Biotek).

[0035] Protein freezing solution A: conventional protein freezing solution plus 2% stabilizer (v / v);

[0036] Protein freezing solution B: conventional protein freezing solution plus 5% stabilizer (v / v);

[0037] Protein freezing solution C: conventional protein freezing solution plus 10% stabilizer (v / v).

[0038] Randomly select 3 protein numbers 1, 2, and 3 from the protein library, divide the samples into 15 tubes at 50 μg / ml for each protein, divide them into 3 groups, add protein freezing solutions A, B, and C, and store at -20°C After storage for a period of ti...

Embodiment 3

[0043]Fish gelatin 15-25g / L, Tris 4.526-5.045g / L, sodium edetate 14-22g / L, trehalose 25-30g / L, Tween-80 3-6g / L . Biological preservative Proclin 300 0.5 ~ 2.0g / L, use double distilled water to prepare a solution in proportion, and filter to sterilize. BCA protein concentration determination kit (purchased from Biyuntian, product number: P0011). Microplate reader (ELx808 Biotek).

[0044] Protein freezing solution: conventional protein freezing solution plus 5% stabilizer (v / v).

[0045] Randomly select 3 protein numbers 1, 2, and 3 from the protein library, divide the samples into 15 tubes at 50 μg / ml for each protein, divide them into 3 groups, add protein freezing solution, and store at 4°C, 25°C, and 37°C After storage for a period of time, the protein concentration was determined according to the method specified in the BCA protein concentration determination kit.

[0046] Table 4 Effect of temperature on protein freezing after adding stabilizer

[0047]

[0048] It...

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Abstract

The invention discloses a biological product stabilizer containing fish gelatin, which comprises fish gelatin, trishydroxymethylaminomethane, sodium edetate, trehalose, Tween-80, and biological preservative Proclin 300. The biological product stabilizer containing fish gelatin has simple formula, low price, wide application range, no safety hazard and good stabilizing effect. The stabilizer can effectively enhance the stability of proteins, antibodies, and test substances in samples, and can also be used as a special stabilizer for ELISA. When used in ELISA technology, it can not only ensure the stability of each core reagent component, but also ensure the accuracy and stability of the results.

Description

technical field [0001] The invention relates to the technical field of biological material preservation, in particular to a fish gelatin-containing stabilizer for biological products such as conventional proteins, antigens, antibodies, and biological detection and diagnostic kits. Background technique [0002] With the continuous development of biotechnology, biological products have played a major role in the diagnosis and treatment of diseases, and the pursuit of the essence of life by human beings. However, some biological products such as conventional proteins, antigens, antibodies, and biological detection and diagnostic kits are easily affected by external factors and lose their activity. This requires an excellent protective agent to reduce the impact of external environmental changes on such biological products. [0003] As the last link of the "central dogma", protein is the most important structural material of organisms and the basis of various life activities. A...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/68
CPCG01N33/6827
Inventor 杨娟李庆项乾刘卫姚雯何峰容李华渊孙颖乐音谱
Owner WUHAN CLOUD CLONE CORP
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