A single-cell gel electrophoresis method for detecting cellular DNA damage

A DNA damage and single-cell gel technology, which is used in measurement devices, material analysis by electromagnetic means, instruments, etc., can solve the problems of complicated operation, unclear sample images, and easy degumming of cells, and achieves low experimental cost and implementation. The process is simple and easy to control, and the sample image is clear.

CN106018529BActive Publication Date: 2019-03-12CHONGQING INST OF GREEN & INTELLIGENT TECH CHINESE ACADEMY OF SCI
6 Cites 0 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Publication Date
2019-03-12

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention provides a kit using single cell gel electrophoresis for detecting cell DNA damage and a method. The method includes gel laying, splitting, unwinding, electrophoresis, neutralizing and washing, drying and dyeing, a fluorescence microscope is used for observing a dyeing result and taking photos, and the damage degree of cell DNA is analyzed and evaluated. As three layers of gel need to be laid on a glass slide during sample preparation, a traditional single cell gel electrophoresis technology has the disadvantages of being complex in operation and prone to degumming, and sample images are unclear; while only one layer of gel needs to be laid on a glass slide, the kit has the advantages of being simple in sample preparing process, low in degumming rate, easy to operate, low in experiment cost and the like.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention relates to the field of biotechnology, in particular to a kit and method for detecting cell DNA damage by single-cell gel electrophoresis. Background technique

[0002] With the accelerated development of China's economy, environmental pollution has become increasingly serious. At present, environmental pollution on a global scale is also receiving more and more attention to human health. Among them, DNA damage caused by environmental pollutants will affect the genetic stability of cells, and may have an impact on offspring, causing miscarriage, stillbirth, teratosis and certain genetic diseases in pregnant women. Since cellular DNA damage can cause disease and threaten human health, it is very important to detect cellular DNA damage and understand whether this damage can cause changes in cellular events.

[0003] In 1976, COOK et al. invented a method for studying cellular DNA damage, that is, single-cell gel electrophoresis (Cook P.R,...

Examples

Embodiment Construction

[0033]Embodiments of the present invention are described below through specific examples, and those skilled in the art can easily understand other advantages and effects of the present invention from the content disclosed in this specification. The present invention can also be implemented or applied through other different specific implementation modes, and various modifications or changes can be made to the details in this specification based on different viewpoints and applications without departing from the spirit of the present invention.

[0034] Human embryonic kidney cells were selected for culture at 37°C, 5% CO 2 , saturated humidity in a carbon dioxide incubator, and when the cells grow to the logarithmic phase, the cells are inoculated in a six-well plate, and 3 mL of culture solution is added (the preparation of the culture solution: add Gibco-1640 respectively according to the volume of the required culture solution) Culture medium, fetal bovine serum and penicil...