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Rapid Breeding Method of Purple Stem Cultivated Seedlings

A purple-stem and fast technology, applied in the field of plant propagation, to reduce the probability of deformed seedlings, achieve large-scale production, and avoid damage

Active Publication Date: 2018-08-10
陈思
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is no report on the tissue culture research of Adenophora

Method used

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  • Rapid Breeding Method of Purple Stem Cultivated Seedlings

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] A method for rapid propagation of purple stem cultivated seedlings, comprising the following steps:

[0054] A. Collect seeds from the vigorous Asteraceae mother tree, put the obtained Asteroides seeds in an aqueous solution containing 100g / L milk tree sap, soak them for 24 hours at 30°C, take them out, rinse them with linear tap water, and then The Adenophora seeds were soaked in natural disinfectant solution for 30 minutes, and finally the Adenophora seeds were rinsed 3 times with sterile water and the surface moisture was removed with sterilized filter paper to obtain explants, wherein,

[0055] The preparation method of the natural disinfectant is as follows: crush 8 parts by weight of Daidai, 8 parts by weight of Melaleuca mellifera, 8 parts by weight of Eucalyptus blueus, 7 parts by weight of calamus, 4 parts by weight of lemongrass and 4 parts by weight of cloves to 50 mesh, Add 500 parts by weight of water for soaking at room temperature for 1 hour, then vacuum ...

Embodiment 2

[0078] A method for rapid propagation of purple stem cultivated seedlings, comprising the following steps:

[0079] A. Collect seeds from the vigorous Asteraceae mother tree, place the obtained Amethystaceae seeds in an aqueous solution containing 100g / L milk tree sap and soak them for 30 hours at 30°C, take them out, rinse them with linear tap water, and then The Adenophora seeds were soaked in natural disinfectant solution for 30 minutes, and finally the Adenophora seeds were rinsed 4 times with sterile water and the surface moisture was removed with sterilized filter paper to obtain explants, wherein,

[0080] The preparation method of the natural disinfectant is as follows: crush 9 parts by weight of Daidai, 9 parts by weight of Melaleuca mellifera, 9 parts by weight of Eucalyptus globulus, 8 parts by weight of calamus, 5 parts by weight of lemongrass and 5 parts by weight of cloves to 80 mesh, Add 500 parts by weight of water to soak at room temperature for 1 hour, then r...

Embodiment 3

[0103] A method for rapid propagation of purple stem cultivated seedlings, comprising the following steps:

[0104] A. Collect seeds from the vigorous Asteraceae mother tree, place the obtained Amethystia seeds in an aqueous solution containing 100g / L milk tree sap, soak them for 36h at 30°C, take them out, rinse them with linear tap water, and then The Adenophora seeds were soaked in natural disinfectant solution for 30 minutes, and finally the Adenophora seeds were rinsed 5 times with sterile water and the surface moisture was removed with sterilized filter paper to obtain explants, wherein,

[0105] The preparation method of the natural disinfectant is as follows: 10 parts by weight of Daidai, 10 parts by weight of Melaleuca mellifera, 10 parts by weight of Eucalyptus blueus, 9 parts by weight of calamus, 6 parts by weight of lemongrass and 6 parts by weight of cloves are pulverized to 100 mesh, Add 500 parts by weight of water for soaking at room temperature for 1 hour, th...

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Abstract

The invention provides a method for quickly breeding cultivation seedlings of stewartia sinensis. The method includes steps of selecting and disinfecting seeds; cultivating aseptic seedlings; inducing multiple shoots; carrying out rooting cultivation; cultivating strong seedlings; cultivating the strong seedlings in seedling beds and the like. Compared with the traditional methods for growing seedlings of stewartia sinensis, the method has the advantages that only few seeds of the stewartia sinensis are required, large quantities of cultivation seedlings can be bred within a short time, excellent hereditary characters of the stewartia sinensis can be kept, seedlings are high in quality, the method is low in malformed seeding rate, the survival rate of the seedlings can reach 95%, the field transplanting survival rate of the cultivation seedlings can reach 80%, scale production and field large-area afforestation growth can be carried out on nursery plants of the stewartia sinensis, and technical support can be provided for effectively protecting the stewartia sinensis which is a rare and endangered plant.

Description

technical field [0001] The invention belongs to the technical field of plant propagation, and in particular relates to a rapid breeding method for purple stem cultivated seedlings. Background technique [0002] Stewartia sinensis (Latin name: Stewartia sinensis Rehd. et Wils.) belongs to the perennial woody plant of the genus Stewartia sinensis in the family Theaceae. Due to the continuous destruction of vegetation and poor natural regeneration, the purple stem plants have been decreasing day by day, and are listed as "endangered species" in the Chinese Red Book. Purple stem is a unique relic plant in China, which is of scientific significance to the study of East Asia-North America flora. Its bark is flaky and detached, the inner bark is brown and yellow, smooth, mottled and beautiful, and the flowers are white and yellow. Courtyard; the material is heavy, durable and beautiful in color, and it is an excellent material for making furniture; the root bark, stem bark, and fr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00A01G17/00A01G24/25A01G24/23A01G24/15A01G24/20A01G24/22A01G24/10A01C1/00A01C1/08A01N65/44A01P1/00A01P3/00
CPCA01C1/00A01C1/08A01G22/00A01G24/00A01H4/001A01H4/008A01N65/00A01N65/44A01N2300/00Y02A40/22Y02P60/40
Inventor 陈思
Owner 陈思
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