Method for assisting in identifying sheep growth characters based on rs430810656 SNP locus

A technology for growth traits and auxiliary identification, which is applied in the field of auxiliary identification of sheep growth traits, and can solve the problems of ten or even decades of time and slow sheep

Active Publication Date: 2016-10-26
INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
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  • Abstract
  • Description
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Problems solved by technology

The selection and improvement of sheep using traditional breeding methods has achieved remarkable results, which has promoted the improvement of local sheep populations and the formation of new

Method used

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  • Method for assisting in identifying sheep growth characters based on rs430810656 SNP locus
  • Method for assisting in identifying sheep growth characters based on rs430810656 SNP locus
  • Method for assisting in identifying sheep growth characters based on rs430810656 SNP locus

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Experimental program
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Effect test

Embodiment 1

[0057] Embodiment 1, SNP site and genotyping

[0058] A number of primer pairs were designed for each exon of the sheep TRHDE gene, the regulatory region of 1000 bp upstream of the sheep TRHDE gene and the regulatory region of 1000 bp downstream of the sheep TRHDE gene. SNP loci and the association analysis between SNP loci and different traits.

[0059] Through the above-mentioned large number of experiments, the specific primer pairs were obtained as follows:

[0060] F (sequence 1 of the sequence listing): 5'-TCAGTTGTCAGAGCAAAGTGA-3';

[0061] R (SEQ ID NO: 2 of the Sequence Listing): 5'-TCTCGTCTTGTTTCTCTCAGT-3'.

[0062] Among the 343 Ujumqin sheep test groups, blood DNA samples of 30 individuals were selected to form a mixed pool of DNA at the same concentration, PCR amplification was performed using primer pairs composed of F and R, and the PCR amplification products were sequenced . Analysis of the sequencing profile, combined with the genome-wide association analys...

Embodiment 2

[0066] Embodiment 2, association analysis

[0067] 1. Association analysis of SNP loci and growth traits of four-month-old sheep

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Abstract

The invention discloses a method for assisting in identifying sheep growth characters based on an rs430810656 SNP locus. The method comprises the following step of detecting whether the genetype of the rs430810656 SNP locus in genome DNA of a sheep to be detected is AA genetype or GG genetype or GA genetype, wherein the growth character of the sheep with the AA genetype is superior to that of the sheep with the GA genetype, and the growth character of the sheep with the GA genetype is superior to that of the sheep with the GG genetype. The invention further provides a specific primer pair. The specific primer pair is composed of a primer F and a primer R which are as shown in a first sequence of a sequence list and a second sequence of the sequence list respectively. The method can be used for assisting in screening the sheep with good growth characters, has important significance in breeding of the sheep and has important significance in further research of the growth characters of the sheep.

Description

technical field [0001] The invention relates to a method for assisting the identification of growth traits of sheep based on the rs430810656 SNP site. Background technique [0002] SNP (single nucleotide polymorphism) mainly refers to the polymorphism of DNA sequence caused by the variation of a single nucleotide at the genome level, including transition, transversion, insertion or deletion. SNP, which is densely distributed in the genome, is considered to be the most promising genetic marker because of its huge amount of genetic information. The detection of human SNP markers has become an important means of genetic diagnosis. The research on the genetic map of SNP markers has greatly promoted the molecular assisted breeding, and the genes of many important traits in agricultural animals have been mapped. [0003] Sheep are livestock with important economic traits in the world and in my country, and provide a large amount of meat, wool, skin and other products for human be...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6869C12Q1/6888C12Q2600/124C12Q2600/156
Inventor 张莉马晓萌杜立新轩俊丽魏彩虹赵福平朱才业吴明明袁泽湖刘瑞凿
Owner INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
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