Application of Cryptotanshinone in Preparation of Tumor Medicine

A cryptotanshinone, experimental technology, applied in the application field of cryptotanshinone in the preparation of tumor drugs, can solve the problem of not many studies on the mechanism of action, and achieve the effect of increasing phosphatase activity

Inactive Publication Date: 2019-01-11
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, its anti-tumor activity has been gradually recognized. Studies have found that CTS has obvious inhibitory effects on various human tumors such as prostate cancer, glioma, and lymphocytic leukemia, which has attracted widespread attention. There are not many researches on the mechanism of action, and it still needs to be further explored from the aspects of molecular mechanism and influence on cell signaling pathways

Method used

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  • Application of Cryptotanshinone in Preparation of Tumor Medicine
  • Application of Cryptotanshinone in Preparation of Tumor Medicine
  • Application of Cryptotanshinone in Preparation of Tumor Medicine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1I

[0021] Example 1 IGF-1 induces cell proliferation

[0022] Add final concentrations of 1, 3, 10, 30, 100 μg·L –1 IGF-1 treated PC12 cells and hRPE cells. 10 μL of IGF-1 was added to each well, and the same volume of DMEM medium was added to the control group. 3-5 replicate wells were set up for each concentration. Placed at 37°C, 5% CO 2 After culturing in the cell culture box for 24 h, the medium was aspirated, and MTT assay was performed to determine the effect of IGF-1 on the proliferation of PC12 cells and hRPE cells.

[0023] MTT test results such as figure 1 and figure 2 shown, from figure 1 It can be seen from the figure that after different concentrations of IGF-1 act on PC12 cells, compared with the blank group, IGF-1 can significantly promote cell proliferation in a dose-dependent manner. The concentration of IGF-1 was 3 μg·L –1 has promoted the proliferation of PC12 cells ( P –1 , the promoting effect on cell proliferation has been extremely significant ( ...

Embodiment 2

[0025] Example 2 Inhibitory effect of cryptotanshinone on cell proliferation induced by IGF-1

[0026] In order to investigate whether CTS can inhibit the proliferation of PC12 cells induced by IGF-1, we first added CTS (20 μM) to pretreat PC12 cells for 40 min, and then added IGF-1 (10 μg / L) to act on PC12 cells for 24 h, and MTT The effect of CTS on IGF-1-induced cell proliferation was detected by the method. At the same time set only 10 μg·L –1 A control group treated with IGF-1; a control group treated with CTS only (20 μM); and a control group cultured without IGF-1 and CTS. 3-5 replicate wells were set up for each concentration. Placed at 37°C, 5% CO 2 After culturing in the cell incubator for 24 h, the medium was sucked off, and MTT assay was performed to investigate the effect of CTS on IGF-1-induced cell proliferation.

[0027] MTT test results such as image 3 As shown, it can be seen that compared with the blank control group, the IGF-1 treatment group has a s...

Embodiment 3

[0028] Example 3 Inhibitory effect of cryptotanshinone on IGF-1R signaling pathway

[0029] 1) The effect of CTS on the phosphorylation levels of IGF-1R, PI3K / Akt and Ras / Erk proteins induced by IGF-1

[0030] In order to study the effect of CTS on the phosphorylation of IGF-1R and its downstream PI3K / Akt and Ras / Erk induced by IGF-1 stimulation, we intervened PC12 cells with CTS, followed by IGF-1 treatment for 10 min. The samples were collected, and the phosphorylation levels of IGF-1R, Akt and Erk were detected by Western Blot, and the dose-effect curve and time-effect curve of the phosphorylation levels of IGF-1R, Akt and Erk changed with CTS treatment were prepared.

[0031] Western Blot results such as Figure 4 As shown, it can be seen from it that PC12 cells were treated with different concentrations of CTS for 40 minutes, and as the concentration of CTS increased, the phosphorylation level of IGF-1R gradually decreased in a dose-dependent manner. The phosphorylation...

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Abstract

The invention discloses the application of cryptotanshinone in the preparation of tumor drugs. The invention finds that cryptotanshinone (CTS) can significantly inhibit the phosphorylation level of tumor cell IGF-1R. This inhibition is mediated at least in part by the phosphatase SHP2. After interfering with the expression of these phosphatases SHP2, the inhibitory effect of CTS on the phosphorylation level of IGF‑1R could be reversed. The present invention also finds that CTS can increase the phosphorylation level of SHP2 in a time- and dose-dependent manner; the phosphatase activity of SHP2 increases after CTS treatment, and these findings are of great significance for the effective treatment of tumors.

Description

technical field [0001] The invention relates to the application of cryptotanshinone in the preparation of tumor medicine. Background technique [0002] Cryptotanshinone (CTS) is one of the active components of Danshen, a traditional Chinese medicine for promoting blood circulation and removing blood stasis. Its unique chemical structure and pharmacokinetic properties make it gradually become the object of people's attention. It has a wide range of pharmacological effects such as scavenging oxygen free radicals, anti-infection, and improving microcirculation. Studies have confirmed that it has good prospects in antibacterial and anti-inflammatory, metabolic disorders, and neurodegenerative diseases. Treatment of inflammation and cardiovascular system diseases. In recent years, its anti-tumor activity has been gradually recognized. Studies have found that CTS has obvious inhibitory effects on various human tumors such as prostate cancer, glioma, and lymphocytic leukemia, whic...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/58A61P35/00
CPCA61K31/58
Inventor 郑文华孟茜周旋和苏肖英廖日房
Owner SUN YAT SEN UNIV
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