A kind of separation method of squalene and vitamin E
A separation method and technology for squalene, applied in the field of separation of squalene and vitamin E, can solve the problems of high separation cost, unsatisfactory purity, low recovery rate and the like, and achieve low recovery cost and reusable performance. Stable, mild separation process conditions
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Examples
Embodiment 1
[0024] Dissolve 1.135g of β-CD (1mmol) in deionized water to make a saturated solution, place it in a water bath at 35°C and stir, after the β-CD is completely dissolved, mix 0.0822g of squalene and 0.0860g of vitamin E The solution was added dropwise to the aqueous solution of β-CD. The reaction was performed at 180 rpm for 6 h, and then the reactant was frozen at 4°C for 24 h, vacuum filtered, and the filter cake was washed with deionized water and n-hexane, and dried in vacuum at low temperature. The inclusions were extracted by solvent extraction. At room temperature, the inclusions were added to 25ml of absolute ethanol for extraction, stirred for 2 hours under magnetic stirring, the extract was filtered, and the purity was obtained by vacuum distillation. Higher squalene and natural vitamin E have a purity of 87% and 83% through HPLC analysis, and a separation factor of 4.001.
Embodiment 2
[0026] Dissolve 1.135g β-CD (1mmol) in deionized water to make a saturated solution, place it in a water bath at 45°C and stir, after the β-CD is completely dissolved, mix 0.0822g squalene and 0.0860g vitamin E The solution was added dropwise to the aqueous solution of β-CD. The reaction was performed at 180 rpm for 6 h, and then the reactant was frozen at 4°C for 24 h, vacuum filtered, and the filter cake was washed with deionized water and n-hexane, and dried in vacuum at low temperature. The inclusions were extracted by solvent extraction. At room temperature, the inclusions were added to 25ml of absolute ethanol for extraction, stirred for 2 hours under magnetic stirring, the extract was filtered, and the purity was obtained by vacuum distillation. Higher squalene and natural vitamin E can be analyzed by HPLC with a purity of 84% and 79%, and a separation factor of 3.495.
Embodiment 3
[0028] Dissolve 1.135g β-CD (1mmol) into deionized water to make a saturated solution, place it in a water bath at 45°C and stir, after β-CD is completely dissolved, mix 0.1233g squalene and 0.1290g vitamin E The solution was added dropwise to the aqueous solution of β-CD. The reaction was performed at 180 rpm for 6 h, and then the reactant was frozen at 4°C for 24 h, vacuum filtered, and the filter cake was washed with deionized water and n-hexane, and dried in vacuum at low temperature. The inclusions were extracted by solvent extraction. At room temperature, the inclusions were added to 25ml of absolute ethanol for extraction, stirred for 2 hours under magnetic stirring, the extract was filtered, and the purity was obtained by vacuum distillation. Higher squalene and natural vitamin E can be analyzed by HPLC with a purity of 81% and 77%, and a separation factor of 3.415.
PUM
Property | Measurement | Unit |
---|---|---|
separation factor | aaaaa | aaaaa |
purity | aaaaa | aaaaa |
separation factor | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com