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Construction method and application of prostate neuroendocrine carcinoma genetic engineering animal model

A neuroendocrine cancer and genetic engineering technology, applied in the field of constructing a genetically engineered animal model of prostate neuroendocrine cancer, can solve the problems of long research cycle, restricting the development of anti-prostate neuroendocrine cancer drugs, slow development of the pathological process of prostate cancer, etc. achieve high reliability

Active Publication Date: 2016-11-16
GUANGDONG PHARMA UNIV
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Problems solved by technology

[0004] Although spontaneous prostate cancer genetically engineered mice such as TRAMP are currently used for the development of prostate cancer therapeutic drugs, the pathological process of prostate cancer in model mice develops slowly, resulting in a long research cycle, which seriously restricts the development of anti-prostate neuroendocrine cancer drugs. Development

Method used

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  • Construction method and application of prostate neuroendocrine carcinoma genetic engineering animal model
  • Construction method and application of prostate neuroendocrine carcinoma genetic engineering animal model
  • Construction method and application of prostate neuroendocrine carcinoma genetic engineering animal model

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Embodiment 1

[0024] One, TRAMP; PSGL-1 - / - The establishment of hybrid mice (see the construction process figure 1 ): The male PSGL-1 - / - Mice and female TRAMP mice are raised in co-cage, each cage is bred with the best ratio of male:female=1:3, and the F1 generation will be separated into cages for identification; TRAMP in the F1 generation will be positive; PSGL-1 + / - Female mice and male PSGL-1 - / - F2 generation mice were obtained by crossing mice, and F2 generation was divided into cages; TRAMP in F2 generation was positive; PSGL-1 - / - Female mice with PSGL-1 - / - Male mice are crossed and expanded to obtain F3 generation mice, and F3 generation will be divided into cages to identify the TRAMP; PSGL-1 - / - Mice are used in experiments.

[0025] Among them, TRAMP and PSGL-1 - / - The identification method of mice is as follows:

[0026] Mouse genomic DNA extraction: put the tissue in a labeled EP tube; immerse the tissue in 500ul lysis buffer A (25mM NaOH 0.5g; 0.2mM Na 2 EDTA.2H 2 O0.037g plus H ...

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Abstract

The invention discloses a construction method of a prostate neuroendocrine carcinoma genetic engineering animal model. The construction method includes the following steps that a TRAMP mouse with the PSGL-1 gene knocked off is raised to 24 week old or more to obtain the prostate neuroendocrine carcinoma genetic engineering animal model. The model more naturally simulates the generation and development process of clinical diseases at the overall level, and complex biological problems and drug action are effectively combined to study the treatment effect of drugs. In addition, various background factors in the human body can be considered by carrying out study in the mouse model live animal constructed by use, and research conclusions are high in reliability. Therefore, it is found that the experiment mouse model is an effective tool for screening the drugs for resisting prostate neuroendocrine carcinoma and is of important significance in finding key targets of the effective treatment drugs and treatment methods of nerve diseases.

Description

Technical field [0001] The present invention relates to the technical field of disease animal model preparation, in particular to a method for constructing a genetically engineered animal model of prostate neuroendocrine cancer and its application. Background technique [0002] Prostate cancer is a common genitourinary system tumor in men, which seriously affects men's physical health and causes great psychological pressure on patients. The incidence and mortality of prostate cancer account for the first place among male malignant tumors. In recent years, the incidence of prostate cancer in my country has shown a significant upward trend. Clinically, the main treatment methods for prostate cancer patients include surgery, radiotherapy, chemotherapy, and endocrine therapy. Surgical treatment is currently the first choice for cancer treatment, but most patients with prostate cancer are diagnosed at an advanced stage, and often miss the best time for surgery, and radical prostatic ...

Claims

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Application Information

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IPC IPC(8): A01K67/02A61K49/00
CPCA01K67/02A61K49/0008
Inventor 章倩倩王丽京段有发
Owner GUANGDONG PHARMA UNIV
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