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Electrochemiluminescence wash buffer

A buffer solution and electrochemical technology, which is applied in the field of preparation and composition of chemiluminescent cleaning buffer solution, can solve the problems of storage, transportation and inconvenient use

Active Publication Date: 2017-12-19
GUANGZHOU DONGLIN BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, since sodium azide is a well-known dangerous chemical reagent, there are many inconveniences in storage, transportation, and use. Therefore, it is extremely important to find alternative reagent formulations

Method used

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  • Electrochemiluminescence wash buffer
  • Electrochemiluminescence wash buffer
  • Electrochemiluminescence wash buffer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Components of Chemiluminescent Wash Buffer:

[0018] Sodium chloride 1g / L, polydextrose 1g / L, Proclin 300 0.1g / L, Tween 20 0.2g / L, Tris T-1378 1.0g / L, Tris-HCl 1.0g / L, 3-mercaptoindole 0.5g / L, lanthionine 0.5g / L, solution pH 8.20.

Embodiment 2

[0020] Components of Chemiluminescent Wash Buffer:

[0021] Sodium chloride 7g / L, polydextrose 3g / L, Proclin 300 0.8g / L, Tween 20 0.3g / L, Tris T-1378 0.7g / L, Tris-HCl 0.4g / L, 3-mercaptoindole 0.4g / L, lanthionine 0.4g / L, solution pH 8.35.

Embodiment 3

[0023] Components of Chemiluminescent Wash Buffer:

[0024] Sodium chloride 5g / L, polydextrose 2g / L, Proclin 300 0.6g / L, Tween 20 0.5g / L, Tris T-1378 0.5g / L, Tris-HCl 0.6g / L, 3-mercaptoindole 0.3g / L, lanthionine 0.4g / L, solution pH 8.40.

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Abstract

The invention relates to an electrochemiluminescence cleaning buffer suitable for a Backman full-automatic immunoassay analyzer, and the electrochemiluminescence cleaning buffer is used in combination with an Access immunoassay system and a specific Access immunity test reagent for diluting and cleaning a sample and providing buffer environment for the whole system, and has a strong practical value and prospect of industry.

Description

technical field [0001] The invention relates to a preparation formula of a chemiluminescent cleaning buffer that can be used for a Beckman automatic immune analyzer, and belongs to the field of in vitro diagnostic reagents for medical devices. Background technique [0002] At present, a variety of immunoassay methods are applied in the field of immunoassay, among which chemiluminescence immunoassay (CLIA) is a technology that combines immune reaction and chemiluminescence reaction to detect antigen or antibody. It is to label the luminescent substance or enzyme on the antigen or antibody. After the immune reaction is over, add an oxidant or enzyme substrate to emit light. By measuring the intensity of the emitted light, the concentration of the analyte is determined according to the standard curve. The main advantages of CLIA are high sensitivity, long validity period of markers, wide detection range, and full automation. [0003] Access2 automatic immunoassay analyzer is p...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N35/00
CPCG01N35/00
Inventor 张传春屈兴翠
Owner GUANGZHOU DONGLIN BIOTECH CO LTD
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