Method for detecting toxicity and safety of contaminated mulberry leaves

Abstract

The invention discloses a method for detecting toxicity and safety of contaminated mulberry leaves. Through a mulberry leaf silkworm breeding test, the tested mulberry leaves are used for breeding healthy silkworm larvae, and general toxicity, neurotoxicity, organ toxicity, development toxicity, reproduction toxicity and pathogenic microorganism pathogenicity are tested. Compared with an ordinary detection method, the method is easier, more convenient, rapider and reliable, comprehensive, sensitive and accurate in result, and has high practical value. The toxicity and safety evaluation result obtained through the method can provide reference for safety evaluation of the mulberry leaves as livestock and poultry feed and edible and medicinal products and is used for evaluating silkworm health hazards and breeding risks of the contaminated mulberry leaves as feed. Thus, through the mulberry leaf silkworm breeding biological test, toxicity and evaluation safety are measured, technical routes are feasible, and application prospects are wide.

Description

technical field [0001] The invention belongs to the fields of medicine and food safety and silkworm breeding, and in particular relates to a method for detecting the toxicity and safety of polluted mulberry leaves. Background technique [0002] Mulberry is a perennial woody plant. The mulberry leaves are rich in nutrients. The dry matter contains about 24%-28% of crude protein, 23%-26% of total sugar, and 3.5%-6.0% of crude fat. It is rich in dietary fiber, amino acids, vitamins and A variety of trace elements necessary for animals and human body, containing active ingredients such as alkaloids and flavonoids. Mulberry leaves are the only plant feed for silkworms and the basis for silkworm cocoon production; mulberry leaves can also be used as feed for poultry and livestock. The Ministry of Health lists mulberry leaves as both food and medicine. Mulberry leaf is a traditional Chinese medicinal material, containing active ingredients such as 1-deoxynojirimycin (DNJ) alkaloi...

AI Technical Summary

Problems solved by technology

[0003] The use of chemical pesticides in mulberry gardens and nearby crops will pollute mulberry leaves and contain pesticide residues; biological pesticides such as Beauveria bassiana pesticides, Bacillus thuringiensis pesticides, and nuclear polyhedrosis virus (fungus, bacteria, virus) preparations, etc. Pests in mulberry gardens such as whitefly and mulberry borer will have the same disease as silkworm, and the dead insects, excrement, scales and other polluted mulberry

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