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Preparation method of galactosyl salidroside and derivatives thereof

A technology of salidroside and galactoside, applied in the field of glycoengineering, can solve the problems of time-consuming, cumbersome, single product, etc., and achieve the effects of simple operation, cheap substrate, and simple product separation and purification

Active Publication Date: 2019-07-26
SHANDONG UNIV
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Problems solved by technology

The method is time consuming and cumbersome and involves the use of a range of organic solvents
[0005] Compared with the chemical method, the biological enzyme method can catalyze a one-step reaction to synthesize glycosides. The substrate is simple, the reaction is fast, and the environment is friendly. However, the yield of galactosyl-salidroside reported by fungal-derived enzymes is only 10.9%. , and the product is single

Method used

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  • Preparation method of galactosyl salidroside and derivatives thereof
  • Preparation method of galactosyl salidroside and derivatives thereof
  • Preparation method of galactosyl salidroside and derivatives thereof

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Embodiment

[0052] The preparation method of galactosyl salidroside and derivatives thereof comprises the steps of:

[0053](1) A reaction system with a lactose concentration of 1M, a p-hydroxyphenylethanol concentration of 0.25M, and a β-galactosidase concentration of 0.4mg / mL was prepared using a phosphate buffer solution with a concentration of 50mM and a pH of 7.0;

[0054] The β-galactosidase in the step (1) is the β-galactosidase whose amino acid sequence is shown in SEQ ID NO.1 as described in Chinese patent document CN1786170A (application number 200510044897.9);

[0055] (2) React the reaction system prepared in step (1) at 50°C for 5 minutes, boil at 100°C for 10 minutes to terminate the reaction, centrifuge at 10,000 rpm for 20 minutes, and take the supernatant; HPLC analysis, the The total yield of galactosyl-salidroside and its derivatives produced by the reaction was 50.0%, of which galactosyl-salidroside accounted for 39.4%, and the two derivatives accounted for 10.6%.

[...

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Abstract

The invention relates to a preparation method of galactose type salidroside and a derivative thereof. The method comprises the following steps that 1, a reaction system with the lactose concentration being 0.1-1 M, the p-hydroxyphenylethyl alcohol concentration being 0.05-0.5 M and the beta-galactosidase concentration being 0.004-4 mg / mL is prepared a phosphate buffer; 2, the reaction system reacts for 5-30 min under the condition that the temperature ranges from 30 DEG C to 50 DEG C, a reaction is terminated, solid-liquid separation is conducted, and supernatant is taken; 3, the supernatant is separated and dried, and the galactose type salidroside and the derivative thereof are prepared. According to the method, a substrate is cheap, reacting is rapid, operation is easy and convenient, time and labor are saved, the production cost is low, and the application prospect is wide.

Description

technical field [0001] The invention relates to a preparation method of galactosyl-type salidroside and its derivatives, in particular to a method for synthesizing p-hydroxyphenylethylgalactoside and its derivatives by using β-galactosidase, belonging to the technical field of sugar engineering . [0002] technical background [0003] Salidroside naturally exists in the medicinal plant Rhodiola rosea, and its chemical structure is p-hydroxyphenylethyl-O-β-D-glucoside, which has important pharmacological functions, such as neuroprotection, cardiovascular protection, delaying Anti-aging, anti-fatigue, anti-oxidation, anti-tumor, anti-coagulation, anti-virus, anti-microwave radiation, etc., are added in a small amount in traditional Chinese medicine and skin care products, and have great application potential. At present, it has been found that the galactoside-type salidroside, that is, p-hydroxyphenylethyl-β-D-galactoside, is superior to salidroside in terms of neuroprotection...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07H15/18C12P19/44
CPCC07H15/18C12P19/44
Inventor 卢丽丽齐亭亭肖敏徐莉
Owner SHANDONG UNIV
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