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A kind of phytoplankton specimen embedding agent and its application

A technology of phytoplankton and embedding agent, applied in the field of embedding agent, can solve the problems of semi-permanent marine phytoplankton specimens that are easy to deform and do not involve marine phytoplankton cells, and achieve good optical permeability and not easy to crack.

Active Publication Date: 2019-07-26
国家海洋局南海环境监测中心
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0012] In summary, the currently disclosed methods are mainly used for large and medium-sized biological specimens or human tissue specimens that are easy to fix and whose cell contents can be relatively intact, or for improving the clarity of the microscope. A method for preparing specimens that retains the characteristics of marine phytoplankton cells for a long time, and the semi-permanent marine phytoplankton specimens are not suitable for the preparation of semi-permanent marine phytoplankton specimens due to their small size, simple cells, and easy deformation once fixed; and , and because organic matter such as chromophores is easily decomposed by microorganisms, there is currently no prior art that can be used for the preparation of semi-permanent marine phytoplankton specimens

Method used

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  • A kind of phytoplankton specimen embedding agent and its application
  • A kind of phytoplankton specimen embedding agent and its application
  • A kind of phytoplankton specimen embedding agent and its application

Examples

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Embodiment 1

[0044] An embodiment of the phytoplankton specimen embedding agent of the present invention, the phytoplankton specimen embedding agent described in this embodiment is made up of the following components by volume: 5 parts of fixative, 200 parts of corn syrup, 95 parts of diluent ( That is: the volume ratio of fixative to the sum of fixative and diluent is fixative: (fixative+diluent)=5:100, the volume ratio of corn syrup to the sum of fixative and diluent is corn syrup: (fix agent+diluent)=100:50); wherein, the fixative is glutaraldehyde, the corn syrup is malt syrup, and the diluent is water.

[0045] The preparation method of the phytoplankton specimen embedding agent described in this example is as follows: first dissolve the fixative in the diluent in proportion to obtain the aqueous solution of the fixative, and then add the corn syrup in proportion to the aqueous solution of the fixative to obtain the plankton. Plant specimen embedding agent.

Embodiment 2

[0047] An embodiment of the phytoplankton specimen embedding agent of the present invention, the phytoplankton specimen embedding agent described in this embodiment is made up of the following components by volume: 15 parts of fixative, 100 parts of corn syrup, 85 parts of diluent ( That is: the volume ratio of fixative to the sum of fixative and diluent is fixative: (fixative+diluent)=15:100, the volume ratio of corn syrup to the sum of fixative and diluent is corn syrup: (fix agent+diluent)=100:100); wherein, the fixative is paraformaldehyde, the corn syrup is malt syrup, and the diluent is water.

[0048] The preparation method of the phytoplankton specimen embedding agent described in this embodiment is the same as that in Embodiment 1.

Embodiment 3

[0050] An embodiment of the embedding agent for phytoplankton specimens of the present invention, the embedding agent for phytoplankton specimens described in this embodiment is made up of fixative, corn syrup and diluent; the fixative is 10 volume parts, and the diluent is 90 volume parts Part (that is: the volume ratio of the sum of fixative and fixative and diluent is fixative: (fixative+diluent)=10:100); the volume ratio of the sum of the corn syrup and fixative and diluent is Corn syrup: (fixative+diluent)=100:150; wherein, the fixative is glutaraldehyde, the corn syrup is malt syrup, and the diluent is water.

[0051] The preparation method of the phytoplankton specimen embedding agent described in this embodiment is the same as that in Embodiment 1.

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Abstract

The invention relates to an embedding medium and an application of the embedding medium, in particular to a phytoplankton specimen embedding medium and an application of the phytoplankton specimen embedding medium. The phytoplankton specimen embedding medium comprises a fixing agent, grain syrup and a diluent, wherein a volume ratio of the fixing agent to the fixing agent and the diluent is (5-15):100; and a volume ratio of the grain syrup to the fixing agent and the diluent is 100:(50-150). The phytoplankton specimen embedding medium cannot destroy phytoplankton cells, and has better optical permeability; phytoplankton cell information can be reserved completely in an optical microscope; and the embedding medium provides a foundation for accurate morphological identification and classification.

Description

technical field [0001] The invention relates to an embedding agent and an application thereof, in particular to an embedding agent for a phytoplankton specimen and an application thereof. Background technique [0002] In recent years, with the extensive development of marine environmental monitoring business, the state has paid more and more attention to the quality management of marine environmental monitoring. In order to test the technical capabilities and levels of marine phytoplankton detection by various marine monitoring agencies, improve the quality of marine ecological environment monitoring data, and strengthen the business exchanges between marine monitoring agencies, the State Oceanic Administration has successively formulated the "National Marine Ecological Environment Monitoring Quality Management for Three Years". Action Plan" (2015-2017), "2016 National Marine Ecological Environment Monitoring Quality Assurance Work Plan", etc. According to the requirements ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N1/36
CPCG01N1/36
Inventor 杨振雄李扬董燕红方宏达娄全胜易斌吕意华杨熙韦桂秋
Owner 国家海洋局南海环境监测中心
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