Mudskipper's multi-type vitellogenin Vtg gene full-length sequence and cloning method thereof

Abstract

The invention discloses mudskipper's multi-type vitellogenin Vtg gene full-length sequence and a cloning method thereof; amino acid sequences of mudskipper's vitellogenin are shown as in SEQ ID NO. 2, or in SEQ ID NO. 4 or in SEQ ID NO. 6; corresponding genes include VtgAa gene, VtgAb gene and VtgC gene. Type-III Vtg gene full-length CDSs (coding sequences) of mudskipper's Vtg family are acquired herein, the blank in mudskipper's Vtg gene family is filled, and the mudskipper's multi-type vitellogenin Vtg gene full-length sequence and the cloning method thereof are significant to the issues, such as more accurate indication for corresponding water area pollution conditions, and deeper research on the protein expression of aquatics in polluted water areas.

Description

technical field [0001] The invention belongs to the field of molecular biology, and in particular relates to a full-length sequence of a mudskipper polymorphic vitellogenin (vtg) gene based on a new mudflat model fish and a cloning method thereof. Background technique [0002] Fish vitellogenin (vitellogenin, Vtg) is a yolk precursor protein synthesized by the liver under the action of estradiol (estradiol-17β, E2) and then secreted into the blood. Normally, Vtg is a female-specific serum protein that appears in the blood of female fish during yolk formation, but male fish and juveniles can also induce the generation of Vtg in vivo under the action of exogenous estrogen. [0003] The mudskipper Periophthalmus modestus is a typical representative species among the fish that live in tidal flats. Mudskippers belong to Perciformes, Goby suborders, Mudskipperidae, and Mudskipper genus. They inhabit brackish waters in estuaries, coastal tidal flats or low tide areas of bottom mud...

AI Technical Summary

Problems solved by technology

However, the traditional detection method for a single type of Vtg does not isolate the corresponding protein and gene for multiple types of Vtg, so the specificity is often poor, and there are even cases where multiple types of Vtg are mixed, and the accuracy of the detection results needs to be improved urgently

[0005] There is no report about the polymorphic vtg gene and its full-length cDNA sequence of the mudskipper

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