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A kind of extract that promotes cholesterol reverse transport and its preparation method and application

An extraction method and extract technology, applied in the field of traditional Chinese medicine, to achieve the effects of promoting bile acid synthesis, lowering blood lipids, and improving the degree of fatty degeneration of liver cells

Active Publication Date: 2020-08-11
ZHEJIANG CHINESE MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the commonly used blood lipid-lowering drugs in clinical practice are mainly chemical drugs such as statins, niacin, fibrates, and polyenes. The effects of chemical drugs on lipid-lowering are certain, but long-term use often causes liver and kidney damage, gastrointestinal reactions, etc. to varying degrees Adverse reactions

Method used

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  • A kind of extract that promotes cholesterol reverse transport and its preparation method and application
  • A kind of extract that promotes cholesterol reverse transport and its preparation method and application
  • A kind of extract that promotes cholesterol reverse transport and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1: Research on the Effect of Different Alcohol Concentrations on the Extraction Ratio of Components such as Aegina Lactone in the Extract of Aegina

[0022] 1. Experimental materials

[0023] 1.1. Chromatographic conditions: Chromatographic instrument: Agilent 1200HPLC instrument, chromatographic column: Ultimate-XB-C 18 (5μm 4.6*250nm), detection wavelength: 235nm, column temperature: 30°C, flow rate: 1ml / min, mobile phase: ultrapure water (A)-acetonitrile (C), gradient elution: 0min, 56%C, 44 %A; 50min, 56%C, 44%A; 55min, 95%C, 5%A; 60min, 95%C, 5%A.

[0024] 1.2. Reference substances: isoagalactone (Lot: 160710), buyalinolactone (Lot: 160421), and buyarolactone (Lot: 160510) were purchased from Beijing Beina Chuanglian Biotechnology Research Institute;

[0025] 1.3. Preparation of different extracts of black yao yao: take appropriate amount of yao yao decoction pieces, respectively add 10BV water, 30%, 50%, 70%, 90% ethanol to reflux extraction twice, each ...

Embodiment 2

[0031] Example 2: Comparative research on components such as araegina lactone in the extracts of argusticoides obtained by different preparation methods

[0032] 1. Experimental materials

[0033] 1.1, chromatographic conditions and reference substance are the same as in Example 1.

[0034] 1.2. Preparation of different extracts of black yao yao: Take appropriate amount of decoction pieces of yao yao yao, and prepare each extract of yao yao according to the following method:

[0035] (1) Add 10BV70% ethanol to the herbal decoction pieces and reflux extraction twice, each time for 1h, filter the filtrate, recover the ethanol under reduced pressure and concentrate to an appropriate volume, extract the concentrate with petroleum ether, ethyl acetate and n-butanol in sequence, collect Each extract, the solvent is recovered under reduced pressure and concentrated, and dried to obtain the extract of black medicine petroleum ether (A), the extract of ethyl acetate (B), the extract o...

Embodiment 3

[0040] Example 3: Study on the blood lipid-lowering effect of the extract of Wuyao alcohol extraction and water precipitation

[0041] 1. Experimental materials

[0042] 1.1. Animals Male SD rats, weighing 200±20 g, were purchased from the Experimental Animal Center of Zhejiang Academy of Medical Sciences.

[0043] 1.2. Drugs and reagents are provided by Tiantaishan Wuyao Co., Ltd.; add 10BV70% ethanol to reflux extraction of black medicine pieces for 2 times, each time for 1 hour, filter the filtrate, recover ethanol under reduced pressure and concentrate to 1 / 3 volume, concentrate Add 3-6 times of water, stir, centrifuge, separate the precipitate, and dry under reduced pressure to obtain the alcohol-extracted water-sedimented extract of wuyao. Before use, take an appropriate amount of the above-mentioned sample, and prepare it with pure water to prepare a liquid of corresponding concentration for future use. Positive control: atorvastatin. TC, TG, LDL-C, HDL-C, AST, ALT an...

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Abstract

The invention discloses an extract product capable of promoting reverse transport of cholesterol and a preparation method and an application thereof. The extract product comprises 26-40 mg / g of isolinderalactone, 15-23 mg / g of linderane, and 19-30 mg / g of lindenenol. The lindera aggregate part is obtained by alcohol extracting water precipitating, has the effect for promoting reverse transport of cholesterol, and can be used for preparing the medicines for adjusting blood fat and preventing, improving and treating atherosclerosis and non-alcoholic fatty liver disease. The lindera aggregate part is extracted from Zhejiang medicinal material lindera aggregate having reputation of elixir of life, and the extract product has the advantages that the resource is abundant, the hypolipidemic effect is obvious, exploitation of the relative product is promoted, and the extract product brings good social and economic benefits.

Description

technical field [0001] The present invention relates to the technical field of traditional Chinese medicines, in particular to a part of black yao yao which can significantly promote the reverse transport of cholesterol, which is prepared by alcohol extraction, water sedimentation and centrifugation, and its preparation for preventing, improving and treating hyperlipidemia. Drugs for atherosclerosis, atherosclerosis and non-alcoholic fatty liver disease. Background technique [0002] Hyperlipidemia (Hyperlipidemia, HLP) is a metabolic disease in which one or several lipid structures in plasma are unbalanced due to abnormal lipid metabolism in the body. HLP is the primary risk factor for atherosclerosis (AS). The occurrence of cerebrovascular diseases is directly related, and the cardiovascular and cerebrovascular diseases that occur on the basis of AS rank among the top deaths. Targeted treatment of abnormal lipid metabolism can effectively reduce the incidence of periphera...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/54A61P9/10A61P1/16A61P3/06
CPCA61K36/54A61K2236/333A61K2236/53
Inventor 楼招欢张光霁鲁文杰
Owner ZHEJIANG CHINESE MEDICAL UNIVERSITY
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