Total internal reflection fluorescence microscopy imaging device based on ellipsoid mirror

Abstract

The invention discloses an ellipsoidal reflector-based total internal reflection fluorescence microscopic imaging device and belongs to the field of optical microscopic illumination. In the device, ring light modulated via an anti-dazzling screen is adopted and diverged after being focused via an illumination object lens, light rays are focused on a second focal point of an ellipsoidal reflector after being reflected via the ellipsoidal reflector, a large apex angle hollow cone light beam which focused at a first focal point of an ellipsoid face is generated, and therefore total internal reflection fluorescence imaging can be realized; when illumination light energy remains unchanged, an energy utilization rate can be greatly improved via the imaging device, illumination intensity can be enhanced, light rays coming from any illumination direction can be reflected via the ellipsoidal reflector, illuminating light rays that illuminate an angles range from zero degree to 360 degrees can be generated, shadow free illumination can be achieved, interference fringes of coherent light can be weakened, a signal to noise ratio of images can be increased, and imaging quality can be improved.

Description

technical field [0001] The total internal reflection fluorescent microscopic imaging device based on the ellipsoid mirror of the present invention belongs to the field of optical microscopic imaging, and has wide applications in the research fields of life science, physical chemistry, material science and the like. Background technique [0002] With the discovery of new mechanisms in the field of life sciences and the synthesis of new substances in the field of materials science, optical microscopes have developed rapidly, and some high-tech technologies have continued to make breakthroughs. A large number of facts in life sciences prove that the dynamic characteristics of cells originate from the aggregation and interaction of individual protein molecules. To this end, it is necessary to vigorously develop super-resolution imaging technology, so as to detect the details of various life activities of cells at the molecular scale, and further reveal the process of life activi...

AI Technical Summary

Problems solved by technology

[0004] Aiming at the occlusion problem, the commonly used rotating optical wedge method can focus the incident light on the back focal plane of the objective lens, thereby generating a hollow light cone and achieving symmetrical illumination in all directions, but the incident angle cannot be adjusted

In addition, symmetrical illumination can also be achieved by using the W-shaped reflector method, but the adjustment range of the incident angle is relatively small

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