PIK3CA gene mutation detection primer probe and kit thereof
A detection kit and detection primer technology, applied in the biological field, can solve the problems of difficulty in simultaneous detection of multiple sites, limited length, and difficulty, and achieve the effects of avoiding adverse effects, avoiding inhibition, and reducing costs.
Inactive Publication Date: 2017-07-28
上海赛安生物医药科技股份有限公司
View PDF2 Cites 8 Cited by
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Due to the limitation of hardware equipment, it is difficult to realize simultaneous detection of multiple sites, and the dPCR multiplex
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View moreImage
Smart Image Click on the blue labels to locate them in the text.
Smart ImageViewing Examples
Examples
Experimental program
Comparison scheme
Effect test
Login to view more PUM
Login to view more Abstract
The invention relates to a PIK3CA gene mutation detection primer probe and a kit thereof. The kit comprises a digital PCR premixed liquid for preparing a digital PCR reaction liquid, a drop stabilizer and a primer probe mixed liquid; the primer probe mixed liquid comprises upstream and downstream primers A and a mutation probe A for detecting the E542K site of the 542nd codon, upstream and downstream primers B and a mutation probe B for detecting the E545K site of the 545th codon, upstream and downstream primers C for detecting the 1047th codon, a probe C for detecting the H1047L site of the 1047th codon and a probe D for detecting the H1047R site of the 1047th codon. The PIK3CA gene mutation detection kit is high in sensitivity and specificity and small in quantity demand of samples, and can perform multiple PCR detection quickly, conveniently and accurately.
Description
technical field [0001] The invention relates to a primer, a probe and a detection product for detecting PIK3CA gene mutation, belonging to the field of biotechnology. Background technique [0002] Studies have found that PIK3CA amplification, deletion, and somatic mutations exist in a variety of human cancers (colon cancer, breast cancer, brain cancer, liver cancer, gastric cancer, and lung cancer, etc.). It is closely related to the occurrence and development of the tumor, and it is also closely related to the stage, grade and prognosis of the tumor. Therefore, detection of PIK3CA mutations in different stages of tumor patients is of great significance for judging the prognosis of patients [0003] Exploring the mechanism of PIK3CA drug resistance and predicting the prognosis of patients has become a feasible way. Now it is clear that tumor cells will release DNA into the circulation system, so that the specific nucleic acid sequences of early and advanced tumor patients c...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more Application Information
Patent Timeline
Login to view more IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6886C12Q1/686C12Q2600/106C12Q2600/118C12Q2600/156C12Q2537/143C12Q2563/107C12Q2563/159
Inventor 赵新泰王明赵会
Owner 上海赛安生物医药科技股份有限公司
Who we serve
- R&D Engineer
- R&D Manager
- IP Professional
Why Eureka
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Social media
Try Eureka
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap