Determination method for HPLC specific chromatogram of codonopsis tubulosa kom dried root

Abstract

The invention discloses a determination method for the HPLC specific chromatogram of codonopsis tubulosa kom dried root. According to the present invention, codonopsis tubulosa kom dried roots from more than 10 different production places are collected, and are respectively prepared into samples, the HPLC maps and the UV spectrograms of the samples and a reference substance lobetyolin are obtained, and after the lobetyolin peak is determined as the reference peak, the HPLC reference specific chromatogram of the codonopsis tubulosa kom dried root is constructed by using a traditional Chinese medicine chromatographic fingerprint similarity evaluation system so as to provide the basis for the effective identification of the codonopsis tubulosa kom dried root from other codonopsis pilosula herbs having the sibling species and provide a set of feasible and effective scheme for the quality control of the codonopsis tubulosa kom dried root; and the determination method has characteristics of simpleness, easy performing, and good use effect.

Description

technical field [0001] The invention relates to the field of pharmacy, in particular to a method for determining the HPLC characteristic spectrum of Guizhou Codonopsis pilosula. Background technique [0002] Guizhou Codonopsis "Quality Standards for Chinese Medicinal Materials and Ethnic Medicinal Materials in Guizhou Province" (2003 Edition) is a collection variety, which is the dried root of Codonopsis tubulosa Kom. It has the functions of tonifying the middle and benefiting qi, strengthening the spleen and benefiting the lungs, etc. It is used for the symptoms of weak spleen and stomach, shortness of breath and heart palpitations, lack of food and loose stools, asthenia and cough, internal heat and thirst. It is mainly distributed in the central and western parts of Guizhou and some southern areas, especially in the northwest. It is a famous authentic medicinal material in Guizhou. Because of its strong body, firm texture and not heavy wood, it has aroma, strong sweetness...

AI Technical Summary

Problems solved by technology

However, in recent years, due to disorderly excavation, serious damage to the ecological environment and other reasons, the amount of wild resources has declined sharply. The existing wild resources are far from meeting the needs of today's market. To evaluate the quality of the medicinal materials, the quality control level is low, and the quality of medicinal materials varies, which seriously affects the healthy and sustainable development of related industries

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