A microarray chip, preparation method, kit and detection method for detecting protein expression in cell stress response

Abstract

The invention discloses a microarray chip for detecting protein expression in a cell stress reaction and its preparation method, kit and detection method. The microarray chip comprises seven stress reaction markers, can ensure inner cell protein expression change corresponding to a cell stress reaction under different stimulation conditions and realizes fast screening and evaluation. A hydroformylation-modified glass sheet is used as a solid carrier so that specific antibody bonding is stable and activity is high. The microarray chip can effectively eliminate the false positive and false negative results of the spectral chip test. The cross-reaction test result shows that the microarray chip has high index detection sensitivity and good specificity. The preparation method has simple processes, realizes fast detection, finishes the whole process in 2d, has high sensitivity and good specificity, realizes a low production cost and has a wide industrialization prospect.

Description

technical field [0001] The invention relates to the field of chip detection, more specifically, to a microarray chip for detecting protein expression in cell stress response, a preparation method, a kit and a detection method. Background technique [0002] Cellular stress is a series of adaptive changes that occur when prokaryotic or eukaryotic cells suffer from various obvious environmental changes or encounter radiation, reactive oxygen species, etc., resulting in macromolecular damage, which eventually leads to changes in gene expression, enhancing the ability of cells to resist damage and Survivability under adverse conditions. Currently, common cellular stress processes mainly include apoptosis, autophagy, cell proliferation, cell migration, cell stemness, and protein translation. [0003] Different cellular stress responses to different external stimuli are mainly reflected in differences in intracellular protein expression. For example, when cells are starved, in or...

AI Technical Summary

Problems solved by technology

However, there are many difficulties that need to be overcome in the development of protein chips: 1. The basis of protein chips is antigen-antibody affinity reaction. Stable situation; 2. The abundance of different proteins in clinical samples is different, and the affinity of antibody-antigen reaction is different. Improper sample dilution is prone to false negative situations; 3. There are cross-reactions between proteins, which seriously affect the detection The accuracy of the results, as the number of detection antibodies increases, the more difficult it is to overcome cross-reactions

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