A method of measuring generation toxicity of medicine and personal nursing products using caenorhabditis elegans, relates to a method of measuring generation toxicity of medicine and personal nursing products (PPCPs) using caenorhabditis elegans. After being contaminated on different concentration gradients and exposure time by synchronized caenorhabditis elegans, life, hatching time, generation time, body size, body corner frequency, inverse motion and olfactory stimulation of caenorhabditis elegans in parental generation P0, first filial generation F1 and second fillial generation F2 are measured, and the result is compared. These indexes characterize toxic effect of PPCPs from different sides, to estimate generation toxicity. The invention is convenient in operation and storage, little in apparatus, provides instruction for measurement and forecast for PPCPs toxicity in real environment from two sides of exposure dose and exposure time, also provides fast estimating approach for procedure of PPCPs, actual effect judgement of bursting event handling, and zoology risk of the novel PPCps.