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Method for storing live trichodina

A preservation method and technology of trichomonas, applied in the direction of protozoa, etc., can solve problems such as trichomoniasis research troubles and research interruptions, and achieve the effects of fast reproduction, long preservation time, and simple operation

Inactive Publication Date: 2017-10-20
WUHAN ACADEMY OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since the onset period of trichomonas is concentrated in April-July, the collection and research on trichomonas is mainly concentrated in April-July, and the supply of diseased fish will be interrupted, which will lead to the interruption of its research, which will bring great harm to the trichomonas. research has caused great distress

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  • Method for storing live trichodina
  • Method for storing live trichodina
  • Method for storing live trichodina

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Embodiment Construction

[0023] The present invention will be further described in detail below in conjunction with the accompanying drawings, so that those skilled in the art can implement it with reference to the description.

[0024] It should be noted that the experimental methods described in the following embodiments, unless otherwise specified, are conventional methods, and the reagents and materials, if not otherwise specified, can be obtained from commercial sources; in the description of the present invention, The terms "landscape", "portrait", "top", "bottom", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", The orientation or positional relationship indicated by "inner", "outer", etc. is based on the orientation or positional relationship shown in the drawings, which is only for the convenience of describing the present invention and simplifying the description, and does not indicate or imply that the referred device or element must have Certain orientations, co...

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Abstract

The invention discloses a method for storing live trichodina. The trichodina and hydra are co-cultured according to an amount ratio of (5 to 20):1. The co-culture method specifically comprises the following steps of S1, feeding the trichodina and the hydra into a transparent storage container with water according to an amount ratio of (5 to 20):1, sealing an opening of the storage container by an air-permeable material, and ensuring that each hydra corresponds to the 3-5cm<2> water surface; S2, feeding daphnia into the storage container at the interval of two days, wherein each hydra corresponds to four daphnia; S3, counting the trichodina and the hydra at the interval of 24h, and maintaining the amount ratio to be (5 to 20):1. The method has the advantages that the operation is simple, the repeatability is strong, the storage time of trichodina is long, the storage amount of trichodina is large, and the storage method can be widely applied to the testing and research of the trichodina.

Description

technical field [0001] The invention relates to the research field of microbial living preservation, more specifically, the invention relates to a method for preserving living organisms of trichomonas. Background technique [0002] Trichomonas can parasitize various freshwater fish and cause various trichotillomaniasis. Trichotiliasis occurs in all regions of the country and throughout the year. The harm caused by trichomonas occurs frequently from April to July, during which the water temperature is maintained at around 20 to 28°C. The large number of trichomonas breeds and causes the fry and fingerlings of various freshwater fish to be infected. In severe cases, it can cause a large number of fry and fingerlings die. Trichomonas are transmitted by contact, and Trichomonas that leave fish can transfer hosts through various media such as water and aquatic organisms. The main factors causing the outbreak of trichotillomaniasis are poor water quality, insufficient food, ove...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/10
Inventor 张立强艾桃山喻运珍邓平周伟东郑瑞珠张生元丁桂珍魏朝辉罗杨志李勤
Owner WUHAN ACADEMY OF AGRI SCI
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