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Organic-cauliflower plant doubling method

A technology of pine cauliflower and plants, which is applied in the field of doubling of pine cauliflower plants, can solve the problems of low doubling rate and achieve the effect of increasing the doubling rate

Inactive Publication Date: 2017-11-24
张家港市松田创新农业科技有限公司
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AI Technical Summary

Problems solved by technology

[0002] Most of the existing plant doubling techniques are root immersion in colchicine solution, and the doubling rate is low

Method used

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Experimental program
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Embodiment Construction

[0011] The technical scheme of the present invention will be further elaborated below.

[0012] Above-mentioned a kind of pine cauliflower plant doubling method comprises the following steps:

[0013] (1) Cultivate the pine cauliflower embryoid body in the first differentiation medium;

[0014] (2) Adding colchicine to the first differentiation medium, so that the embryoid body of Songhuacai is cultured in the first differentiation medium for 2-4 days, in this embodiment, the culture time is 3 days;

[0015] (3) Taking out the embryoid body of Songhuacai in the first differentiation medium, and putting it into the second differentiation medium without colchicine for culturing.

[0016] By adding colchicine to the first differentiation medium to cultivate the embryoid body of Songhuacai, and then putting the embryoid body of Songhuacai into the second differentiation medium without colchicine, the growth rate of Songhuacai was improved. The doubling rate of regenerated plants...

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Abstract

The invention discloses an organic-cauliflower plant doubling method. Through adding colchicine into a differentiation culture medium to carry out culture on organic-cauliflower embryoid and then putting the organic-cauliflower embryoid into a differentiation culture medium which does not contain the colchicine to carry out the culture, the doubling rate of an organic-cauliflower regenerated plant is increased, and is increased to 75 percent or above from original 45 percent.

Description

technical field [0001] The invention relates to a method for doubling a pine cauliflower plant. Background technique [0002] Most of the existing plant doubling techniques are the root soaking method with colchicine solution, and the doubling rate is relatively low. Contents of the invention [0003] The purpose of the present invention is to provide a method for doubling the plants of Songhuacai, which can increase the doubling rate of the regenerated plants of Songhuacai from the original 45% to 75% or more. [0004] In order to achieve the above object, the technical scheme adopted in the present invention is: [0005] A kind of doubling method of pine cauliflower plant, comprises the following steps: [0006] (1) Put the pine cauliflower embryoid body into the first differentiation medium; [0007] (2) Adding colchicine to the first differentiation medium, and culturing the pine cauliflower embryoid bodies in the first differentiation medium for 2-4 days; [0008]...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/08A01H4/00
CPCA01H1/08A01H4/005
Inventor 杨继峰
Owner 张家港市松田创新农业科技有限公司
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