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A kind of sweet corn haploid chemical soaking and doubling method

A sweet corn and haploid technology, applied in the field of agriculture, can solve the problems of lack of sweet corn research, achieve the effects of reducing chemical consumption, reducing toxicity, and saving seed treatment time

Active Publication Date: 2017-02-01
CROP RES INST GUANGDONG ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Relevant research has been relatively comprehensive and in-depth in common corn, but research on sweet corn is very lacking

Method used

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  • A kind of sweet corn haploid chemical soaking and doubling method
  • A kind of sweet corn haploid chemical soaking and doubling method
  • A kind of sweet corn haploid chemical soaking and doubling method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Example 1 Chemical soaking seed doubling test of "Yuetian No. 13" sweet corn haploid seeds doubling in 0.02% colchicine at 32°C for different times

[0044] (1) Seed disinfection

[0045] Divide sweet corn haploid seeds into 9 portions, each containing 450 haploid seeds, and put them in sterile mesh bags; sterilize them with 75% ethanol for 1 min, take them out, and then use the volume fraction Treat with 15% sodium hypochlorite solution for 15 minutes, and then rinse with sterile water for 3 times;

[0046] (2) Double the seed

[0047] Put 9 parts of sterilized seeds into the nutrient solution containing mass fraction of 0.02% colchicine, and the amount of nutrient solution added to each seed mesh bag shall be subject to just submerging the seeds; 3 parts of the seeds are placed in a 32°C Treat in an artificial climate box for 18 hours, place 3 seeds in an artificial climate box at 32°C for 24 hours, and place the other 3 seeds in an artificial climate box at 32°C fo...

Embodiment 2

[0060] Example 2 Chemical soaking doubling test of haploid seeds of "Yuetian No. 13" sweet corn in 0.02% colchicine at 27°C for different times

[0061] (1) Seed disinfection

[0062] Divide sweet corn haploid seeds into 9 portions, each containing 450 haploid seeds, and put them in sterile mesh bags; sterilize them with 75% ethanol for 1 min, take them out, and then use the volume fraction Treat with 15% sodium hypochlorite solution for 15 minutes, and then rinse with sterile water for 3 times;

[0063] (2) Double the seed

[0064]Put 9 parts of sterilized seeds into a nutrient solution containing 0.02% colchicine in mass fraction, the amount of nutrient solution added to each seed mesh bag shall be subject to just submerging the seeds, and 3 parts of the seeds shall be placed in a 27°C Treat in an artificial climate box for 18 hours, place 3 seeds in an artificial climate box at 27°C for 24 hours, and place the other 3 seeds in an artificial climate box at 27°C for 30 hour...

Embodiment 3

[0076] Example 3 Chemical Soaking Doubling Test of Haploid Seeds of "Yuetian No. 13" Sweet Corn in 0.02% Colchicine at 22°C for Different Times

[0077] (1) Seed disinfection

[0078] The haploid seeds of sweet corn were equally divided into 9 portions, and each portion contained 450 haploid seeds, which were respectively packed in sterile mesh bags. Disinfect with ethanol with a volume fraction of 75% for 1 min, take it out, and then treat it with a sodium hypochlorite solution with a volume fraction of 15% for 15 min, and then rinse it with sterile water for 3 times;

[0079] (2) Double the seed

[0080] Put 9 parts of sterilized seeds into the nutrient solution containing 0.02% colchicine in mass fraction, the amount of nutrient solution added to each seed mesh bag is subject to just submerging the seeds, and 3 parts of the seeds are placed in a 22 ℃ Treat in an artificial climate box for 18 hours, place 3 seeds in an artificial climate box at 22°C for 24 hours, and place...

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Abstract

The invention relates to the technical field of the agriculture and specifically relates to a chemical speed-soaking haploid doubling method for sweet corn. The method comprises the following specific steps: performing surface sterilization on sweet corn seeds, next putting the sterilized seeds in a nutrient solution containing colchicine for doubling treatment, then sowing the seeds and transplanting, wherein the doubling treatment is carried out in the nutrient solution containing 0.02wt% of colchicine at 32 DEG C for 30 hours, in the nutrient solution containing 0.02wt% of colchicine at 32 DEG C for 24 hours or in the nutrient solution containing 0.06wt% of colchicine at 32 DEG C for 30 hours. The method is low in mechanical damage, convenient to operate, low in phytotoxicity, and low in toxic action on a human body; the average doubling rate of the method is 11.82%-14.29%, which is 6-7 times higher than a traditional doubling method and a natural doubling method, so that the method is convenient for large-scale application.

Description

technical field [0001] The invention relates to the field of agricultural technology, in particular to a method for doubling sweet corn haploid seeds by chemical soaking. Background technique [0002] Sweet corn grains contain rich and balanced nutrients and have a good taste, and have become one of the indispensable nutritional and health foods. Haploid technology is the preferred method for maize breeding because of its short breeding cycle, high efficiency and high purity of inbred lines. [0003] At present, haploid breeding technology is widely used in common corn, but there are few reports on sweet corn. One of the main obstacles restricting haploid breeding is the low doubling rate of haploids. There are many influencing factors. In addition to the genetic background of the doubling material, temperature, humidity, time, timing, concentration and type of reagents all significantly affect the doubling rate. magnification. The relevant research has been relatively co...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/08
Inventor 胡建广李高科陈新振李春艳
Owner CROP RES INST GUANGDONG ACAD OF AGRI SCI
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