A method for doubling maize haploids using herbicides

Abstract

The invention belongs to the technical field of plant breeding, and discloses a method for doubling corn haploids by using herbicides, comprising: the corn hybrid Guidan 0810 is used as the female parent, and the induced line CAU5 is used as the male parent to hybridize the female parent; the female parent is pollinated The ear of the material is mature, harvested and dried, and corn haploid kernels are selected from the ear; field identification of the authenticity of the haploid corn plant: sowing the haploid corn kernels, and selecting the real corn haploid plant; doubling the corn by the dripping method Haploid and foliar spraying method doubled corn haploid; the ears of corn haploid plants were bagged with sodium sulfate paper bags before silking, and artificial self-pollination was carried out; after the pollination period, the corn single The number of ploidy plants was calculated and the doubling rate of maize haploid was calculated. The operation method of the invention is simple and convenient, which is beneficial to protect the health of operators and reduce the harm to the environment.

Description

technical field [0001] The invention belongs to the technical field of organic phosphorus compound-containing biocides, pest repellents or attractants, or plant growth regulators, and in particular relates to a method for doubling corn haploids by using herbicides. Background technique [0002] At present, the existing technology commonly used in the industry is as follows: the breeding of new corn varieties is the main factor to promote the development of corn production. For example, in the process of China's corn production growth, the increase in per unit yield contributed 68.4% to the growth of total corn production (Dai Jingrui, 2010). In the process of breeding new maize varieties, the creation of inbred lines is the core link. The two-ring or one-ring method for breeding maize inbred lines requires 6-8 generations of self-breeding, which is time-consuming and inefficient. With the development of technology, the combination of traditional breeding methods is still t...

AI Technical Summary

Problems solved by technology

Breeding maize inbred lines by the two-ring or one-ring method requires 6-8 generations of self-breeding, which takes a long time and is inefficient.

[0004] (1) The tassels of existing maize haploid plants do not produce fertile pollen due to sterility, and the doubling rate of tassels is low, which brings difficulties to the application of maize haploid breeding technology

[0005] (2) The currently used colchicine is highly toxic, and the treatment of colchicine waste liquid is also more complicated. If it is not handled properly, it will cause potential harm to personal safety and the environment. After poisoning, it will cause nausea, vomiting, diarrhea, abdominal pain, Gastrointestinal reactions are other symptoms, and even cause cancer. Severe poisoning can cause death and bring harm to the operator's body.

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