Method for doubling corn haploid by herbicides

Abstract

The invention belongs to the technical field of plant breeding and discloses a method for doubling corn haploid by herbicides. The method includes: hybridizing a corn hybrid species Guidan 0810 serving as a female parent and an inducer CAU5 serving as a male parent; after pollination and maturity of female parent cobs, harvesting, drying, and selecting corn haploid kernels from the cobs; performing corn haploid plant authenticity field authentication, namely sowing the corn haploid kernels, and picking real corn haploid plants; adopting a core dripping method and a foliage atomizing method fordoubling the corn haploid; bagging female ears of the corn haploid plants with sodium sulfate paper bags before silking, and performing artificial self pollination; after a pollination stage is finished, counting loose-pollen corn haploid plants, and calculating the corn haploid doubling rate. The method is convenient and simple in operation and beneficial to protection of physical health of operating personnel and reduction of environmental hazards.

Description

Technical field [0001] The invention belongs to the technical field of biocides, pest repellents or attractants containing organic phosphorus compounds, or plant growth regulators, and in particular relates to a method for doubling corn haploids by using herbicides. Background technique [0002] At present, the existing technology commonly used in the industry is as follows: the breeding of new maize varieties is the main factor to promote the development of maize production. For example, in the process of China's corn production growth, the increase in unit yield contributed 68.4% of the total corn output growth (Dai Jingrui, 2010). In the process of breeding new maize varieties, the creation of inbred lines is the core link. The two-ring system or one-ring system method for breeding maize inbred lines requires 6-8 generations of inbred processes, which is time-consuming and low in efficiency. With the development of technology, the combination of traditional breeding methods ...

AI Technical Summary

Problems solved by technology

Breeding maize inbred lines by the two-ring or one-ring method requires 6-8 generations of self-breeding, which takes a long time and is inefficient.

[0004] (1) The tassels of existing maize haploid plants do not produce fertile pollen due to sterility, and the doubling rate of tassels is low, which brings difficulties to the application of maize haploid breeding technology

[0005] (2) The currently used colchicine is highly toxic, and the treatment of colchicine waste liquid is also more complicated. If it is not handled properly, it will cause potential harm to personal safety and the environment. After poisoning, it will cause nausea, vomiting, diarrhea, abdominal pain, Gastrointestinal reactions are other symptoms, and even cause cancer. Severe poisoning can cause death and bring harm to the operator's body.

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