A culture method for expanding hematopoietic stem cells in vitro and enhancing their homing ability

Abstract

The invention discloses a culture method for in-vitro expansion of hematopoietic stem cells and homing ability thereof. The culture method includes steps: (1), adopting a culture medium A to culture the hematopoietic stem cells for 10-20 days, wherein the culture medium A is obtained by adding growth factors like TPO, Il6, SCF and Flt3 into a basal culture medium; (2), after the above culturing procedures, separating out 50% cultured cells, and adopting a culture medium B to culture the cultured cells for 1-12 days to obtain HPC culture liquid, wherein the rest cultured cells are HSC culture liquid, and the culture medium B is obtained by adding growth factors TPO, IL6, SCF and Flt3 into the basal culture medium; (3), mixing the HPC culture liquid with the HSC culture liquid, culturing, and centrifuging to harvest cells. The growth factors are combined with micromolecular compounds to culture and expand the hematopoietic stem cells, so that number of hematopoietic progenitor cells is increased, glycosylation is adopted to improve homing ability of the hematopoietic stem cells, and transgenic technology is not applied, so that the culture method is high in safety.

Description

technical field [0001] The invention relates to a culture method for hematopoietic stem cells, in particular to a culture method for expanding hematopoietic stem cells in vitro and enhancing their homing ability, and belongs to the technical field of cell culture. Background technique [0002] Haematopoietic stem cells (HSCs) are the first adult stem cells to be studied in detail, forming all downstream cells of the blood lineage system (eg, figure 1 shown). HSC exists in the fetal liver tissue in the early stage of fetal development; after the individual is formed, it mainly exists in the bone marrow space. In the physiological state of a healthy adult, hematopoietic stem cells need to replace 10 10 ~10 11 number of blood cells. [0003] In 1956, E.Donnall Thomas used bone marrow transplantation from identical twin brothers to treat leukemia in identical twin individuals. In 1968, the first allogeneic hematopoietic stem transplantation was successful. To date, HSCs ar...

AI Technical Summary

Problems solved by technology

The disadvantages are: 1. Some methods for culturing and expanding HSCs can only expand hematopoietic progenitor cells or short-term hematopoietic stem cells; 2. The program that focuses on cultivating medium-term hematopoietic stem cells ignores the effect of hematopoietic progenitor cells on the recovery of hematopoietic function. 3. Some programs involve the cultivation of HSC cells after genetic modification, and it is difficult to enter the clinic through regulatory approval; 4. HSC derived from umbilical cord blood is difficult to homing and implantation

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