Chip-level three-dimensional dynamic drug detection system, culture device and use method thereof

Abstract

The invention relates to a chip-level three-dimensional dynamic drug detection system, a culture device and a use method thereof. The culture device comprises a gradient concentration generation module and an artificial tumor invasion module; the gradient concentration generation module conveys a culture solution into the artificial tumor invasion module; the artificial tumor invasion module comprises an inner cancer cell culture chamber, an annular normal cell culture chamber and an annular vascular endothelial chamber which are concentrically arranged; all the chambers are spaced by columnar bulges in an array manner, and gaps among the columnar bulges can block the flow of an extracellular matrix without affecting cell migration; the culture solution conveyed by the gradient concentration generation module flows along the annular vascular endothelial chamber to provide nutrients for normal cells and cancer cells in a form of osmotic flow, and wastes generated by the normal cells and the cancer cells are taken away in a form of osmotic flow. The chip-level three-dimensional dynamic drug detection system provided by the invention highly and bionically stimulates the growth environment of the cancer cells in a human body, is used for cancer cell migration ability study and anti-cancer drug screening and is good in effect.

Description

technical field [0001] The invention relates to a chip-level three-dimensional dynamic drug detection system, a culture device and a use method. Background technique [0002] With the development of society and the improvement of living standards, the average life expectancy of human beings has been greatly extended, and tumors are more common in the elderly. Therefore, cancer has increasingly become a worldwide problem that seriously plagues human health. According to the data released by the International Union Against Cancer, the annual number of cancer deaths in the world is as high as 7.6 million. In order to meet the needs of human tumor migration research and drug detection and screening, human normal cells and cancer cells are co-cultured to establish a flexible and reliable method in vitro. , low-cost tumor invasion model is of great significance. [0003] Photocuring 3D printing is to input the object to be printed into the computer control unit in STL format, and...

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Problems solved by technology

[0005] In recent years, some progress has been made in the co-culture of cancer cells and normal cells at home and abroad, but the use of different chambers to culture two kinds o

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