Method for preparing transparent skeleton specimen of small animal, and specimen prepared with method

A technology for transparent bones and small animals, applied in the preservation, application, animal husbandry, etc. of human or animal bodies, which can solve the problems of low yield, unclear staining of specimens, and inability to preserve specimens for a long time.

Inactive Publication Date: 2018-01-12
张乘月
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, there are few literatures on the preparation methods of transparent bone specimens of small animals. The existing methods have many uncertainties, the yield is low, and the preparation process takes a long time, the staining of the obtained specimens is unclear, the transparency is low, and the specimens cannot be preserved for a long time. And other disadvantages, especially for dyeing hard bones, dangerous reagents such as concentrated sulfuric acid are usually used, which cannot be generally applied, and the process conditions need to be further improved

Method used

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  • Method for preparing transparent skeleton specimen of small animal, and specimen prepared with method
  • Method for preparing transparent skeleton specimen of small animal, and specimen prepared with method
  • Method for preparing transparent skeleton specimen of small animal, and specimen prepared with method

Examples

Experimental program
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Effect test

Embodiment 1

[0049] Preparation of transparent bone specimens of common goldfish

[0050] Treat ordinary domestic goldfish with warm water at 40-50°C, peel off scales, rinse with running water, and immerse in absolute ethanol for a week to harden the fish body. Immerse in potassium dichromate solution (0.2mol / l) for about 48 hours to remove fat, then add to absolute ethanol and soak for about 24 hours. Prepare a saturated solution of Alcian blue in 8 times the volume of absolute ethanol and 2 times the volume of glacial acetic acid, and immerse the fish in it for about 24 hours to stain the cartilage. Soak in pure water and change the solution frequently to remove residual solution. Immerse in about 2% potassium hydroxide solution for about 4 hours to make the muscle translucent. Use a syringe to draw potassium hydroxide solution to perform multiple suctions inside the fish to clean the viscera. Prepare a saturated solution of Alizarin Red in absolute ethanol, add 9 times the volume of ...

Embodiment 2

[0052] Preparation of Transparent Skeleton Specimens of Tropical Fish (Pencilfish)

[0053] Adopt the similar method with embodiment 1, but the potassium dichromate solution concentration that adopts is 0.1mol / l, and the potassium hydroxide solution concentration that adopts is 1%.

[0054] The prepared specimens were figure 2 shown.

Embodiment 3

[0056] Preparation of Transparent Skeleton Specimen of Tropical Fish (Goose Fish)

[0057] A method similar to Example 1 was adopted. However, after the fish body hardens, it is immersed in potassium dichromate solution (0.1mol / l) for about 60 hours to remove fat, and then added to absolute ethanol for about 36 hours. Prepare a saturated solution of Alcian blue in 8 times the volume of absolute ethanol and 2 times the volume of glacial acetic acid, and immerse the fish in it for about 36 hours to stain the cartilage. Soak in pure water and change the solution frequently to remove residual solution. Immerse in about 1% potassium hydroxide solution for about 6 hours to make the muscle translucent. Prepare a saturated solution of Alizarin Red in absolute ethanol, add 9 times the volume of 1% potassium hydroxide solution, and soak the fish body for about 48 hours to stain the cartilage.

[0058] The prepared specimens were image 3 shown.

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Abstract

The invention relates to a method for preparing a transparent skeleton specimen of a small animal, and a specimen prepared with the method. According to the invention, muscle of the small animal become transparent, and a colouring agent is adopted for soaking to develop and distinguish different parts of an animal body, so that original position structures of hard bones and cartilages of the animal body are showed directly and accurately. The method is simple in step, the adopted agent is safe and can be used for preparing transparent skeleton specimens of small animals such as goldfishes, tropical fishes, frogs and mice, the prepared specimens can be kept for a long time, structures are showed clearly, and the prepared specimens have relatively high scientific research and teaching values.

Description

technical field [0001] The invention relates to the field of animal specimen production, in particular to a method for preparing small animal transparent bone specimens and specimens prepared by the method. Background technique [0002] Small animal transparent bone specimens are a kind of specimens in which the animal body is processed by anatomical methods, and the muscles of small animals are made transparent by using chemical reagents, so that the stained animal bones can be displayed. The transparent bone specimens of small animals can accurately and intuitively show us the shape and position of the bones of the animal body, just like an X-ray film. Soaking in dyes can develop and distinguish different parts of the animal's body, which is a more intuitive display method than X-ray films. Transparent bone specimens can provide accurate bone structure for scientific research. Compared with traditional stripped bone specimens, they can more accurately show the original po...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/00
Inventor 张乘月
Owner 张乘月
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