An efficient method for separating and extracting uterine stem cells and method for building a library
A technology of uterine stem cells and extraction methods, which is applied in the establishment of uterine stem cell banks, the separation and extraction of uterine stem cells, and can solve the problem of low yield
Active Publication Date: 2021-05-07
上海坤爱生物科技股份有限公司 +1
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[0004] The purpose of the present invention is to provide an efficient method for separating and extracting uterine stem cells and a method for building a bank, so as to solve the defect that the yield of the methods for separating and extracting uterine stem cells in the prior art is low
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[0064] This embodiment provides a specific method step of separating and extracting uterine stem cells and building a bank. Meanwhile, the method step also includes separating and extracting stem cells using the traditional method of separating and extracting uterine stem cells as a comparative example.
[0065] This embodiment includes the following steps:
[0066] Step 1. Collection of menstrual blood
[0067] After the donor signed the informed consent form, the menstrual blood on the second or third day of the menstrual cycle was collected, and 5ml-15ml of menstrual blood collected by the menstrual cup was stored in an equal volume of menstrual blood preservation solution (PBS saline buffered with sodium heparin). solution, and add 100U / ml penicillin and 100U / ml streptomycin), and deliver to the national certified GMP workshop within 48 hours under the condition of 2-8°C.
[0068] Step 2,
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The invention discloses an efficient method for separating and extracting uterine stem cells and a method for building a bank. In the invention, the bottom sediment obtained by centrifuging a menstrual blood sample through density gradient centrifugation is washed and adhered to the wall for culture, and the uterine stem cells are harvested. In the present invention, the number of uterine stem cells obtained by separating the discarded bottom sediment after medium-density gradient centrifugation in the traditional method is about ten times that of the traditional buffy coat, and the uterine stem cell bank can be established after a large number of harvested uterine stem cells are expanded and cultured.
Description
technical field [0001] The invention relates to the field of biomedicine, in particular to the separation and extraction of uterine stem cells and the establishment of a uterine stem cell bank. Background technique [0002] In 1999, "Science" rated the research results of human embryonic stem cells as the top ten scientific and technological progress in the world that year. In 2000, Time magazine listed it as the top ten scientific and technological achievements in the world at the end of the 20th century. Genome will simultaneously become the most promising field of development and application in the new century. So far, stem cell research has become one of the most striking hot spots in the fields of biology and medicine in recent years. Stem cells (SC) are a kind of pluripotent cells with self-renewing ability, which can differentiate into various functional cells under certain conditions. At present, stem cell sources can be divided into embryonic stem cells and adult ...
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IPC IPC(8): C12N5/074C40B50/06
CPCC12N5/0682C40B50/06
Inventor 刘召青陈彦田齐念民
Owner 上海坤爱生物科技股份有限公司