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Breeding method for breeding salt-tolerant rice by radiation mutation and anther culture

A technique for inducing medium and rice, which is applied in botany equipment and methods, applications, plant genetic improvement, etc., can solve the problems of difficult realization, high cost, and little effect, and achieve high efficiency, large genetic gap, and excellent comprehensive traits Effect

Active Publication Date: 2018-02-09
ZHENJIANG AGRI SCI INST JIANGSU HILLY AREAS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the salt damage to rice can be slightly alleviated through reasonable water and soil management and chemical improvement, but it is difficult to achieve because of high cost and little effect

Method used

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  • Breeding method for breeding salt-tolerant rice by radiation mutation and anther culture
  • Breeding method for breeding salt-tolerant rice by radiation mutation and anther culture
  • Breeding method for breeding salt-tolerant rice by radiation mutation and anther culture

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Embodiment 1

[0031] Please combine figure 1 , taking the breeding of salt-tolerant conventional japonica rice as an example, a kind of breeding method of radiation mutagenesis, anther culture and breeding salt-tolerant rice comprises the following steps:

[0032] (1) Select Zhendao 11 with high quality, stripe resistance and multiple spikes as the female parent, and cross with Wu 2329 with high quality and high blast resistance to obtain F 1 , followed by F 1 The female parent was crossed with Xinjing 18 with good taste to obtain a recross combination: Zhendao 11 / Wu 2329 / / Xinjing 18; among them, Zhendao 11, Wu 2329, and Xinjing 18 could all achieve high yield, resistance and quality. Complementary of excellent traits;

[0033] (2) Use gamma ray radiation to induce variation in Zhendao 11 / Wu 2329 / / Xinjing 18, then screen for salt tolerance with 0.5% NaCl solution, soak the seeds for 48 hours, sow, and transplant;

[0034] (3) Select the surviving single-plant Zhendao No. 11 / Wu 2329 / / Xinj...

Embodiment 2

[0040] Please combine figure 2 , taking the breeding of salt-tolerant hybrid japonica rice as an example, a kind of breeding method for radiation mutagenesis and anther culture selection and breeding of salt-tolerant rice comprises the following steps:

[0041] (1) Select the restorer line Yongyou 12 with large spikes and multiple grains as the female parent, and cross it with the restorer line C418 with high affinity, high combining ability and high light efficiency to obtain F 1 , followed by F 1 The female parent was crossed with the high-quality, high-yield, and stripe-resistant restorer line Jiahe 218 to obtain the recross combination Yongyou 12 / C418 / / Jiahe 218; among them, Yongyou 12, C418, and Jiahe 218 were excellent in yield, resistance, quality, and high light efficiency. , Combining ability can realize the complementarity of excellent traits;

[0042] (2) Use gamma ray radiation to induce mutation of Yongyou 12 / C418 / / Jiahe 218, then screen for salt tolerance with...

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Abstract

The invention discloses a breeding method for breeding salt-tolerant rice by irradiation mutagenesis and anther culture. Selected materials for radiation are selected through comprehensive breeding through composite cross of rice varieties which are excellent in comprehensive traits and complementary, and the materials have certain advantages in yield, resistance and quality. Materials selected for anther culture are composite cross species induced by irradiation, and the excellent genetic background of composite cross species diversification provides a wide range of variation sources for inducing salt-tolerant gene mutations, while also providing the basis for stability of other good traits. Based on rooting medium screening including four NaCl concentrations and multiple salt tolerance screening in saline-alkali soil screening, the bred rice is more thorough in salt tolerance; in addition, the salt-tolerant rice is bred through the anther culture which has effects of breaking linkageof salt-tolerant genes and deleterious genes, and the salt tolerance breeding efficiency is higher.

Description

technical field [0001] The present invention relates to the breeding technology of rice, in particular to a kind of breeding method of radiation mutagenesis, anther culture and selection of salt-tolerant rice. Background technique [0002] Rice is the main food crop of our people. With the improvement of people's living standards and the continuous trend of increasing population and decreasing cultivated land, improving rice production and increasing stable supply have been the key issues that my country's rice breeding has been working hard for a long time. Utilizing excellent germplasm resources for high-yield and stable-yield breeding is the most effective and economical way to increase rice yield. [0003] Soil salinization is one of the most serious abiotic stresses that limit crop growth and cause crop yield reduction. Rice planting in saline-alkali land will generally reduce its yield, and even fail to complete growth in severe cases. The area of ​​saline-alkali la...

Claims

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Application Information

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IPC IPC(8): A01H1/06
CPCA01H1/06
Inventor 姚维成景德道余波龚红兵林添资钱华飞孙立亭曾生元李闯杜灿灿胡庆峰
Owner ZHENJIANG AGRI SCI INST JIANGSU HILLY AREAS
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