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A kind of breeding method of radiation mutagenesis, anther culture and selection of salt-tolerant rice

A technique for inducing medium and rice, which is applied in botany equipment and methods, plant genetic improvement, application, etc., can solve the problems of difficult realization, high cost, and little effect, and achieve high efficiency, large genetic gap, and excellent comprehensive traits Effect

Active Publication Date: 2020-05-29
ZHENJIANG AGRI SCI INST JIANGSU HILLY AREAS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, through reasonable water and soil management and chemical improvement, the salt damage to rice can be slightly alleviated, but it is difficult to achieve because of high cost and little effect

Method used

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  • A kind of breeding method of radiation mutagenesis, anther culture and selection of salt-tolerant rice
  • A kind of breeding method of radiation mutagenesis, anther culture and selection of salt-tolerant rice
  • A kind of breeding method of radiation mutagenesis, anther culture and selection of salt-tolerant rice

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Experimental program
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Effect test

Embodiment 1

[0031] Please combine figure 1 , taking the breeding of salt-tolerant conventional japonica rice as an example, a method for breeding salt-tolerant rice by radiation mutagenesis and anther culture, comprising the following steps:

[0032] (1) Select Zhendao 11 with high quality, stripe resistance and multiple ears as the female parent, and cross with Wu 2329 with high quality and high blast resistance to obtain F 1 , followed by F 1 The female parent was crossed with Xinjing 18 with good taste to obtain a recross combination: Zhendao 11 / Wu 2329 / / Xinjing 18; among them, Zhendao 11, Wu 2329, and Xinjing 18 could achieve high yield, resistance and quality. Complementary of excellent traits;

[0033] (2) Gamma ray radiation was used to induce mutations in Zhendao 11 / Wu 2329 / / Xinjing 18, and then screened for salt tolerance with 0.5% NaCl solution, soaked for 48 hours, sowed, and transplanted;

[0034] (3) Select the surviving single-plant Zhendao No. 11 / Wu 2329 / / Xinjing 18 that...

Embodiment 2

[0040] Please combine figure 2 , taking the breeding of salt-tolerant hybrid japonica rice as an example, a method for breeding salt-tolerant rice by radiation mutagenesis and anther culture, comprising the following steps:

[0041] (1) Select the restorer line Yongyou 12 with large spikes and multiple grains as the female parent, and cross it with the restorer line C418 with high affinity, high combining ability and high light efficiency to obtain F 1 , followed by F 1 The female parent was crossed with the high-quality, high-yield, and stripe-resistant restorer line Jiahe 218 to obtain the recross combination Yongyou 12 / C418 / / Jiahe 218; among them, Yongyou 12, C418, and Jiahe 218 were excellent in yield, resistance, quality, and high light efficiency. , Combining ability can realize the complementarity of excellent traits;

[0042] (2) Gamma ray radiation was used to induce the variation of Yongyou 12 / C418 / / Jiahe 218, and then screened for salt tolerance with 0.5% NaCl so...

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Abstract

The invention discloses a breeding method for breeding salt-tolerant rice by irradiation mutagenesis and anther culture. Selected materials for radiation are selected through comprehensive breeding through composite cross of rice varieties which are excellent in comprehensive traits and complementary, and the materials have certain advantages in yield, resistance and quality. Materials selected for anther culture are composite cross species induced by irradiation, and the excellent genetic background of composite cross species diversification provides a wide range of variation sources for inducing salt-tolerant gene mutations, while also providing the basis for stability of other good traits. Based on rooting medium screening including four NaCl concentrations and multiple salt tolerance screening in saline-alkali soil screening, the bred rice is more thorough in salt tolerance; in addition, the salt-tolerant rice is bred through the anther culture which has effects of breaking linkageof salt-tolerant genes and deleterious genes, and the salt tolerance breeding efficiency is higher.

Description

technical field [0001] The invention relates to a rice breeding technology, in particular to a breeding method of radiation mutagenesis and anther culture for breeding salt-tolerant rice. Background technique [0002] Rice is the main food crop of our people. With the improvement of people's living standards and the continuous trend of increasing population and decreasing cultivated land, increasing rice production and increasing stable supply have been the key issues that my country's rice breeding has been working on for a long time. It is the most effective and economical way to increase rice yield by using excellent germplasm resources for high-yield and stable-yield breeding. [0003] Soil salinization is one of the most serious abiotic stresses that limit crop growth and cause crop yield reduction. Rice planting in saline-alkali land will generally reduce its yield, and even fail to complete growth in severe cases. The area of ​​saline-alkali land in the world excee...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/06
CPCA01H1/06
Inventor 姚维成景德道余波龚红兵林添资钱华飞孙立亭曾生元李闯杜灿灿胡庆峰
Owner ZHENJIANG AGRI SCI INST JIANGSU HILLY AREAS
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