Rapid inclination phase error compensation method and apparatus based on wavefront rotation

Abstract

The invention is suitable for the optical imaging correction technology field and provides a rapid inclination phase error compensation method and an apparatus based on wavefront rotation. The methodcomprises the following steps of using coaxial digital holographic microscopic system to collect a hologram of an object to be tested and taking as an original hologram; rotating the original hologramfor 180 degrees clockwise or counterclockwise and acquiring a digital reference hologram which is taken as a rotation hologram; using a Fourier transform method in digital holographic microscopy to carry out phase recovery processing on the original hologram, and acquiring an original expansion phase diagram; using the Fourier transform method in the digital holographic microscopy to carry out phase recovery processing on the rotation hologram and acquiring a rotation expansion phase diagram; and subtracting the original expansion phase diagram from the rotation expansion phase diagram and acquiring a phase diagram after compensation for the original expansion phase diagram. By using the method of the invention, rapid and accurate inclination phase error compensation in coaxial digital holographic microscopy imaging is realized.

Description

technical field [0001] The invention belongs to the technical field of optical imaging correction, in particular to a fast tilt phase error compensation method and device based on wavefront rotation. Background technique [0002] As a quantitative phase measurement technology, digital holographic microscopy can obtain quantitative amplitude information and phase information of the object to be measured with only a single hologram, and has the advantages of non-contact, label-free, high resolution, and low cost. Among them, the coaxial digital holographic microstructure is often used in the quantitative measurement of cells and micro-optical components because of its high time stability and compact characteristics. The characteristic of the coaxial digital holographic microscope system is that the optical path of the object light and the optical path of the reference light go through the same optical path, so this structure can not only maintain the stability of the system, b...

AI Technical Summary

Problems solved by technology

However, the off-axis structure will introduce a tilt phase factor, resulting in a certain angle of tilt error in the reconstruction phase, which affects the accuracy of quantitative phase measurement; the coaxial structure in the coaxial holographic microscope system cannot eliminate the tilt error introduced by the off-axis structure. Tilt errors can only be removed by numerical compensation methods, so in order to obtain accurate object phase information, it is necessary to perform tilt phase error compensation on the reconstructed phase

[0004] In order to eliminate the off-axis tilt in the coaxial digital holographic microscope, someone proposed the spectral center translation method, which translates the spectrum of the virtual image to the center of the spectrum in the hologram spectrum, thereby eliminating the off-axis tilt, but the accuracy of the positioning of the spectral center is limited to the whole pixels, it is necessary to further remove the residual skew distribution

It was proposed to use the numerical compensation method of the phase template to eliminate the off-axis tilt. The phase template can be obtained from the pre-knowledge of the system or the acquisition of a sample-free hologram, but this method needs to know the parameters of the system in advance, or need to acquire two holograms. Suitable for dynamic measurement

Some people propose to use the polynomial fitting method to eliminate the off-axis tilt. This method requires additional numerical fitting calculations, increases the amount of calculations, and is susceptible to noise

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