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Method for detecting antibiotic content in milk

A technology of antibiotics and milk, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of long antibiotic time and high detection cost, and achieve the effects of high sensitivity, low cost, and simple and convenient sample processing

Inactive Publication Date: 2018-02-16
SHANDONG WUZHOU DETECTION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the detection method of antibiotics in milk takes a long time and the detection cost is high

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] In an embodiment of the present invention, a method for detecting antibiotic content in milk comprises the following steps:

[0023] 1) Weigh 3±0.02mL of milk to be tested in a 50mL centrifuge tube, dilute with 20mL of 1% phosphate buffer, vortex to mix, heat in a water bath at 80°C for 5min, cool and centrifuge for 15min at 5000r / min. Pipette the supernatant into a 10mL centrifuge tube; repeatedly extract the residue with 4mL of 1% phosphate buffer, centrifuge and combine the supernatant as a spare solution; the centrifugation speed is 5000r / min.

[0024] 2) Amino solid-phase extraction column, activated with 6mL acetonitrile, pipette the spare solution through the column, collect the effluent, dilute to 5mL with acetonitrile solution, mix well, and filter with 0.22μm microporous nylon filter membrane to prepare the sample to be tested ; 50% acetonitrile solution is a mixed solution of acetonitrile and water, the volume ratio of acetonitrile and water is 1:1.

[0025]...

Embodiment 2

[0030] In an embodiment of the present invention, a method for detecting antibiotic content in milk comprises the following steps:

[0031] 1) Weigh 3±0.02mL of milk to be tested in a 50mL centrifuge tube, dilute with 20mL of 1% phosphate buffer, vortex to mix, heat in a water bath at 75°C for 3min, and centrifuge for 10min after cooling at 5000r / min. Pipette the supernatant into a 10mL centrifuge tube; repeatedly extract the residue with 4mL of 1% phosphate buffer, centrifuge and combine the supernatant as a spare solution; the centrifugation speed is 5000r / min.

[0032] 2) Amino solid-phase extraction column, activated with 6mL acetonitrile, pipette the spare solution through the column, collect the effluent, dilute to 5mL with acetonitrile solution, mix well, and filter with 0.22μm microporous nylon filter membrane to prepare the sample to be tested ; 50% acetonitrile solution is a mixed solution of acetonitrile and water, the volume ratio of acetonitrile and water is 1:1. ...

Embodiment 3

[0038] In an embodiment of the present invention, a method for detecting antibiotic content in milk comprises the following steps:

[0039] 1) Weigh 3±0.02mL of milk to be tested in a 50mL centrifuge tube, dilute with 20mL of 1% phosphate buffer, vortex and mix well, heat in a water bath at 85°C for 8min, and centrifuge for 10-20min after cooling at a speed of 5000r / min. Pipette the supernatant into a 10mL centrifuge tube; repeatedly extract the residue with 4mL of 1% phosphate buffer, centrifuge and combine the supernatant as a spare solution; the centrifugation speed is 5000r / min.

[0040] 2) Amino solid-phase extraction column, activated with 6mL acetonitrile, pipette the spare solution through the column, collect the effluent, dilute to 5mL with acetonitrile solution, mix well, and filter with 0.22μm microporous nylon filter membrane to prepare the sample to be tested ; 50% acetonitrile solution is a mixed solution of acetonitrile and water, the volume ratio of acetonitril...

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PUM

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Abstract

The invention discloses a method for detecting antibiotic content in milk. The method comprises the following steps: weighing 3 minus or plus 0.02 mL to-be-detected milk, putting into a 50mL centrifugal tube, diluting with 20mL of 1% phosphate buffer liquid, carrying out vortex mixing, heating for 3-8 minutes in water bath at the temperature of 75-85 DEG C, cooling, then centrifuging for 10-20 minutes, taking supernatant and transferring into a 10mL centrifugal tube; repeatedly extracting residues with 4mL of 1% phosphate buffer liquid, centrifuging, and then mixing supernatant as ready-to-useliquid; activating an amino solid-phase extraction column with 6mL acetonitrile, taking and transferring the ready-to-use liquid to pass the column, collecting effluent, carrying out constant volumetreatment to 5mL with acetonitrile liquid, uniformly mixing, and filtering, so that a to-be-detected sample is obtained; and carrying out liquid chromatography-tandem mass spectrometry detection. Themethod for detecting antibiotic residues in a milk sample has the advantages of extremely great simplicity and convenience during sample treatment, no technical requirements on operators, high sensitivity, good accuracy, high detection speed and low cost and is applicable to monitoring of multiple antibiotic drug residues in daily milk samples.

Description

technical field [0001] The invention relates to the technical field of food detection, in particular to a method for detecting antibiotic content in milk. Background technique [0002] Antibiotics can not only kill bacteria but also have a good inhibitory and killing effect on mold, mycoplasma, chlamydia, spirochetes, rickettsia and other pathogenic microorganisms. Usually, antibiotics are renamed as antibiotics. Antibiotics can be a substance produced during the growth and reproduction of certain microorganisms. The antibiotics used to treat diseases are not only directly extracted from this, but also completely or partially artificially synthesized. In layman's terms, antibiotics are drugs used to treat various non-viral infections. However, many side effects have been shown in clinical use. The most serious side effect of antibiotics is allergic reaction. Studies have shown that each antibiotic has different degrees of harm to the human body. For example, streptomycin...

Claims

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Application Information

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IPC IPC(8): G01N30/88G01N30/06
CPCG01N30/88G01N30/06
Inventor 尤健孔雪赵雪
Owner SHANDONG WUZHOU DETECTION
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