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A swollen cell virus vsocs/vtk double gene knockout strain and its preparation method and application

A double-gene, gene-based technology, applied in the field of giant cell virus vSOCS/vTK double-gene knockout strain and its preparation, can solve the problems of lack of immune protection from natural infection, difficult promotion and application, and short immune period, and achieve immune Convenient method, excellent protection rate, and high immunity rate

Active Publication Date: 2021-07-30
SUN YAT SEN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, inactivated vaccines are not easy to carry out large-scale production in aquaculture due to short immunization period, large dosage, need for adjuvants, lack of immune protection against natural infection, and need to consume a lot of manpower and material resources. promotion and application

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  • A swollen cell virus vsocs/vtk double gene knockout strain and its preparation method and application
  • A swollen cell virus vsocs/vtk double gene knockout strain and its preparation method and application
  • A swollen cell virus vsocs/vtk double gene knockout strain and its preparation method and application

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Embodiment Construction

[0071] The following description is a preferred embodiment of the present invention, it should be pointed out that for those skilled in the art, without departing from the principle of the present invention, some improvements and modifications can also be made, and these improvements and modifications are also considered Be the protection scope of the present invention.

[0072] The preferred embodiments of the present invention will be described in detail below with reference to the accompanying drawings. The experimental method that does not indicate specific condition in the embodiment, generally according to routine condition. Unless otherwise specified in the examples of the present invention, all reagents and consumables used are commercially available.

[0073] The embodiment of the present invention provides a preparation method of a recombinant viral vaccine with deletion of swollen cell virus vSOCS and vTK genes, comprising the following steps:

[0074] 1. Construc...

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Abstract

The invention provides a vSOCS / vTK double gene deletion strain of mastocyte virus and its preparation method and application, which is an attenuated virus strain produced by deleting the vSOCS gene and vTK gene of the mast cell virus strain. The present invention also provides a mast cell virus vSOCS / vTK double-gene deletion strain with a selection marker and its preparation method and application. The attenuated virus strain made by inserting the first selection marker gene and the second selection marker gene respectively by source recombination. The swollen cell virus vSOCS / vTK double-gene deletion strain provided by the present invention is a recombinant genetically engineered vaccine in which vSOCS and vTK pathogenic genes have been knocked out. Immunization can achieve a significant immune effect, without injection, and has great application value.

Description

technical field [0001] The invention relates to the field of prevention and control of aquaculture diseases, in particular to a double gene knockout strain of swollen cell virus vSOCS / vTK and its preparation method and application. Background technique [0002] Mandarin fish is one of the "Four Famous Freshwater Fishes" in my country, and it is a rare and high-quality economic fish with high economic value. Mandarin fish is popular in the market. While optimistic about the development prospects of mandarin fish aquaculture, the increasingly prominent disease problem has become a bottleneck restricting the development of mandarin fish aquaculture, seriously affecting the enthusiasm of farmers. Its typical example is the iridescent virus cell swelling virus disease of mandarin fish that broke out in our province in 1994, resulting in the mass death of cultured mandarin fish within a week. At present, the disease is still a serious threat to the development of mandarin fish aq...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N7/01C12N15/90C12N15/65A61K39/12A61P31/20C12R1/93
CPCA61K39/12A61K2039/5254C12N7/00C12N15/65C12N15/902C12N2710/00021C12N2710/00034
Inventor 何建国郭长军翁少萍林易凡
Owner SUN YAT SEN UNIV
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