A method for improving the synthesis of polyunsaturated fatty acid oil by Mortierella

A technology of unsaturated fatty acid and Mortierella, which is applied in the field of fermentation engineering, can solve the problems of easy wall hanging of bacterial cells, low yield of polyunsaturated fatty acid oil and low dissolved oxygen concentration, etc., and achieves the effect of good industrial application prospects.

Active Publication Date: 2020-11-13
HUAZHONG UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The technical problem to be solved by the present invention is: to provide a method for improving the synthesis of polyunsaturated fatty acid oil by Mortierella, and to solve the problems of easy wall-hanging, low dissolved oxygen concentration and further increase of the density of bacteria in the existing Mortierella fermentation process limited, resulting in low yields of polyunsaturated fatty acid oils

Method used

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  • A method for improving the synthesis of polyunsaturated fatty acid oil by Mortierella

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] The method for improving the synthesis of polyunsaturated fatty acid oil by Mortierella of the present embodiment comprises the steps:

[0027] 1) Sterilization

[0028] Add the prepared fermentation medium into the fermenter, and steam sterilize the fermenter, fermentation medium, filter device and connected pipelines;

[0029] 2) Aerobic constant temperature culture

[0030] Put the prepared Mortierella alpina seed liquid into the fermentation medium, 100L fermenter, 200rpm, ventilation rate 1.5vvm, 25°C constant temperature culture, monitor the dissolved oxygen concentration during the fermentation process;

[0031] 3) Reflux of oxygen-enriched fermentation supernatant

[0032] When the dissolved oxygen concentration is lower than the saturation percentage of 10% oxygen, the fermented liquid in the fermenter is filtered through a sterile first filter membrane, the pore size of the first filter membrane is 50-200 μm, and the mycelium is left in the fermenter for fer...

Embodiment 2

[0037] The method for improving the synthesis of polyunsaturated fatty acid oil by Mortierella of the present embodiment comprises the steps:

[0038] 1) Sterilization

[0039] Add the prepared fermentation medium into the fermenter, and steam sterilize the fermenter, fermentation medium, filter device and connected pipelines;

[0040] 2) Aerobic constant temperature culture

[0041] Put the prepared Mortierella deepflavus seed liquid into the fermentation medium, 100L fermenter, 400rpm, ventilation rate 0.8vvm, 30°C constant temperature culture, monitor the dissolved oxygen concentration during the fermentation process;

[0042] 3) Reflux of oxygen-enriched fermentation supernatant

[0043] When the dissolved oxygen concentration is lower than the saturation percentage of 20% oxygen, the fermentation liquid in the fermenter is filtered through a sterile first filter membrane, the pore size of the first filter membrane is 50-200 μm, and the mycelium is left in the fermenter fo...

Embodiment 3

[0049] The method for improving the synthesis of polyunsaturated fatty acid oil by Mortierella of the present embodiment comprises the steps:

[0050] 1) Sterilization

[0051] Add the prepared fermentation medium into the fermenter, and steam sterilize the fermenter, fermentation medium, filter device and connected pipelines;

[0052] 2) Aerobic constant temperature culture

[0053] Put the prepared Mortierella Ramanii seed liquid into the fermentation medium, 100L fermenter, 300rpm, ventilation rate 1.0vvm, 27°C constant temperature culture, monitor the dissolved oxygen concentration during the fermentation process;

[0054] 3) Reflux of oxygen-enriched fermentation supernatant

[0055] When the dissolved oxygen concentration is lower than the saturation percentage of 17% oxygen, the fermentation broth in the fermenter is filtered through a sterile first filter membrane, the first filter membrane has a pore size of 50-200 μm, and the mycelium is left in the fermenter for f...

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Abstract

The invention discloses a method for improving the yield of polyunsaturated fatty acids synthesized by using Mortierella. The method comprises the following steps: subjecting Mortierella to normal aerobic constant-temperature culture; when the dissolved-oxygen concentration of fermentation broth is lower than a preset threshold, discharging a part of clear liquid produced after fermentation in a fermentation cylinder into a filter for treatment, introducing air into the discharged clear liquid, and conveying the clear liquid containing saturated air back into the fermentation cylinder throughspraying via a water curtain device, wherein sterile water is used for supplementation if the clear liquid is insufficient; and when the dissolved-oxygen concentration of fermentation broth in the fermentation cylinder has no obvious change and is continuously maintained to be greater than the preset threshold, stopping circulation of the clear liquid, continuing culturing until glucose is completely consumed and stopping fermentation. The method can maintain high thallus concentration, substantially improve the growth rate of thalli and the yield of polyunsaturated fatty acids and overcome the problem of wall hanging of thalli during fermentation. The method is universally applicable to fermentation production of aerobic filamentous fungi and has good industrial application prospects.

Description

technical field [0001] The invention relates to the technical field of fermentation engineering, in particular to a method for improving the synthesis of polyunsaturated fatty acid oil by Mortierella. Background technique [0002] Polyunsaturated fatty acids refer to straight-chain fatty acids containing two or more double bonds and a carbon chain length of 18 to 22 carbon atoms. They have important physiological functions and are widely used in nutrition, medicine and cosmetics, especially for infants. The field of infant food and nutrition. The production of polyunsaturated fatty acids by microbial fermentation has become a research hotspot. Polyunsaturated fatty acids are intracellular products, and the yield is directly related to the biomass of bacteria. Increasing the biomass of bacteria is of great significance for increasing the yield of polyunsaturated fatty acids. Mortierella (Mortierella) is an important strain commonly used in the production of polyunsaturated f...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/64C12R1/645
CPCC12P7/6427
Inventor 余龙江朱圆敏付春华周蓬蓬王晓鹏刘婷婷
Owner HUAZHONG UNIV OF SCI & TECH
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