The invention relates to a method for extracting
grease contained in
mortierella alpine. The method comprises the following steps: (1), inoculating
mortierella alpine strains into a
liquid culture medium for
fermentation to obtain
fermentation liquor; (2), performing filtering to obtain
mortierella alpine wet cells, dissolving the mortierella alpine wet cells in a
sodium chloride solution to obtain a mortierella alpine
cell solution, then performing emulsifying and shearing, and adjusting the temperature to 40-60 DEG C; (3), adjusting the pH value of the mortierella alpine
cell solution to 6.5-7.5, adding an autolysis
inducer, continuously performing stirring, and autolyzing mortierella alpine cells for 8-12 hours in a vacuum
oxygen-free environment to obtain a mortierella alpine autolysis solution; (4), carrying out
centrifugal separation, and collecting upper-layer
grease; (5), collecting lower-layer
solid obtained by
centrifugal separation in the step (4), uniformly mixing the lower-layer
solid with
purified water, performing
centrifugal separation, and collecting upper-layer
grease; and (6), mixing the upper-layer grease collected in the steps (4) and (5). The method can effectively extract the grease in mortierella alpine cells, has the advantages of simple technique and low
energy consumption, and is beneficial to implementing large-scale production.