69 results about "Mortierella bainieri" patented technology

The present invention relates to a process for preparing a biomass, such as from a microbial fermentation, for an extraction process to separate desired chemicals, nutritional products, bioactive components, proteins, carbohydrates, and lipids, from the biomass. Particularly preferred substances to extract include docosahexaenoic acid, docosapentaenoic acid, and arachidonic acid. The present invention also includes extracting the prepared biomass. Biomasses to be treated in accordance with the methods of the invention include plant, animal, and microbial biomass, particularly a microorganism such as Crypthecodinium cohnii and a fungus such as Mortierella alpina.

The invention relates to a recombination gene expression system of mortierella alpina (Mortierella alpina ATCC 32222) and applications thereof. According to the invention, by taking mortierella alpina ATCC 32222 uracil auxotroph strains as materials, a set of recombination gene expression system of mortierella alpina is constructed through carrying out genetic manipulation by using an agrobacterium tumefaciens mediated genetic manipulation technique; and an operation is operated by using the system, so that multiple high-yield polyunsaturated fatty acid mortierella alpina strains are obtained, therefore, the recombination gene expression system of mortierella alpina and construction method and applications thereof disclosed by the invention are of great importance in the basic theory study and product development of oil-producing fungi mortierella alpina ATCC 32222.

The invention relates to a mortierella alpina, M. alpina genetic engineering strain of an overexpression omega 3 desaturase gene (FADS15) and a construction method of the strain. According to the invention, a mortierella alpina, M. alpina uracil nutritional-deficient strain MAU1 (CCFM501) is taken as a material, an agrobacterium tumefaciens-mediated genetic manipulation technology is applied to obtain an omega 3 desaturase (omega 3Des) overexpression strain of high-yield EPA (eicosapentaenoic acid), and the strain and the construction method have important significance on basic theoretical research and product development of mortierella alpina, M. alpine as an oil-producing fungus.

The invention provides a technology for preparing grease containing rich polyunsaturated fatty acid by taking lignocellulose type agricultural and forest wastes as raw materials through fermenting mortierella alpine, aiming at the problems in the prior art. The technology comprises the steps of pre-treating, saccharifying, carrying out mortierella alpine fermentation, extracting the grease and thelike. According to the technology provided by the invention, a fermentation phase of the mortierella alpine adopts a temperature gradual reduction method and the yield of ARA (Arachidonic Acid) can be remarkably improved; the ratio of the ARA in the total grease can reach 48 percent. Compared with the prior art, the technology adopts a cellulose preparation based on fiber-production globule bacteria to realize the saccharifying of lignocellulose, so that the cost of a carbon source and the enzyme utilization cost of a saccharifying phase are remarkably reduced; the cellulose saccharifying efficiency is 80 to 90 percent. Furthermore, a culture medium at a lignocellulose saccharifying phase and a fermentation culture medium in the technology can be circularly utilized, so that water can beremarkably reduced and the dosage of chemicals also can be remarkably reduced; the technology has remarkable effects of reducing wastewater emission and reducing the cost.

The invention discloses a Mortierella elongata PFY capable of utilizing lignocellulose for fermentation production of microbial oil and a method of producing microbial oil by the same. The method mainly comprises the following steps: culturing and screening strains by using a Congo red culture medium; inoculating the strains into a medium containing lignocellulose for culturing; drying and crushing fermented substances; and extracting crude oil in the dried substances through extraction in an organic solvent. According to ITS complete sequence analysis, the screened strains are Mortierella fungi; the strains grow rapidly and can highly efficiently utilize lignocellulose, the utilization rate of lignocellulose is as high as 90% after fermentation is finished, and the dried substances obtained after fermentation contain a great number of microbial oil, wherein the content of unsaturated fatty acid is 59%.

The invention relates to a new separated bacterial strain of mortierella alpine (Mortierella Alpina) and an application thereof. Particularly, the invention relates to CGMCC No.6254, offspring thereof, culture and / or mutant strain and a method and application in preparing arachidonic acid by using the bacterial strain, offspring thereof, culture and / or mutant strain. The separated bacterial strain is a natural bacterial strain, and can stably produce arachidonic acid with a yield as high as 14.32g / L.

The invention relates to a method for promoting production of mortierella alpina and oil yield of arachidonic acid. The method comprises the following steps that fermented clear liquid of the mortierella alpina is collected, and through extraction and concentration of hydrophilic organic solvent, an accelerating agent which can adjust and control the production of the mortierella alpine and the oil yield of the arachidonic aicd is obtained by screening; and in the 0-48 hours at the early stage of fermentation, the accelerating agent is added to a normal medium according to the ratio of 0.1-0.5%, and a conditioned medium is prepared to inoculate and cultured at the temperature of 28 DEG C. After the fermentation, in fermentation results of some embodiments, biomass is 43.2 g / L, oil content is 61.3%, ARA content is 60.7%, ARA yield is 16.1 g / L, and the fermentation period is shortened from 192 hours to 168 hours. The method for promoting the production of the mortierella alpina and the oil yield of the arachidonic acid is simple and effective, promotes the production of the mortierella alpine, improves the oil yield of the arachidonic acid, and is of great significance to industrial application.

The invention discloses a mortierella alpine mutant strain, a method for producing arachidonic acid oil by using the same and arachidonic acid oil. The mortierella alpine mutant strain (Mortierella alpine Y16) is preserved in the China Center for Type Culture Collection (CCTCC) on July 2, 2015, the preservation address is Wuhan University in Wuhan City of China, and the preservation number is CCTCC NO: M2015421. In the arachidonic acid oil produced by using the mortierella alpine mutant strain, the content of triglyceride is more than or equal to 92wt%, the content of arachidonic acid is at least 48wt%, and the content of long-chain saturated fatty acid with more than 20 carbons is lower than 6.3wt%.

The invention provides a mortierella alpina 0901, and an application and microbial inoculums thereof, and belongs to the technical field of microbial inocula. The Latin name of the mortierella alpina0901 is Mortierella alpina, the mortierella alpina is preserved in China General Microbiological Culture Collection Center, and the preservation number is CGMCC No.17791. The mortierella alpina can obviously improve conversion of soil nitrogen, promote nitrogen absorption of plants and improve biomass of plants. The content of indoleacetic acid in maize root systems at a seedling stage is increased by 70% and the biomass of overground parts of maize is increased by 33%, which indicated that mortierella alpina directly promotes crop growth. The content of abscisic acid in root systems is closely related to drought resistance, salt resistance and the like of crops, and the content of the abscisic acid is increased by 36%. Elongation of root systems is promoted, nutrient absorption of the root systems is promoted, the content of nitrogen, phosphorus and potassium in plants is improved, and the yield of crops is increased by 23%.

The present invention relates to an acyl-CoA synthetase homolog protein from microorganisms of the genus Mortierella, a polynucleotide encoding the protein, and so on. The invention provides polynucleotides comprising an acyl-CoA synthetase homolog protein gene from, e.g., Mortierella alpina, expression vectors comprising these polynucleotides and transformants thereof, a method for producing lipids or fatty acids using the transformants, food products containing the lipids or fatty acids produced by the method, etc.

It relates to a Mortierella alpine ATCC32222 uracil auxotroph strain and a construction method thereof. In the present invention, Mortierella alpine ATCC32222 is used as a material and undergoes gene knockout through an Agrobacterium tumefaciens mediated genetic manipulation technology, to obtain the Mortierella alpine uracil auxotroph. The method is of great significance for the basic theoretic researches of the oil producing fungus Mortierella alpine ATCC32222 and product development.

The invention relates to a recombinant Mortierella alpine strain GPD-1 overexpressing G3PD1 and a construction method. According to the invention, uracil-auxotrophic Mortierella alpine MAU1 is used as a material, Agrobacterium tumefaciens is used for mediation, and a G3PD1-overexpressing recombinant strain with obviously increased lipid content is obtained. Compared with wild Mortierella alpine, the total lipid content of the strain provided in the invention is increased by about 50%, and the transcription amount of G3PD1 is increased by about 2 times; so theoretical and application foundations are laid for subsequent industrial application.

The invention discloses a Mortierella alpina mutant strain capable of producing high-yield arachidonic acid, and a fermentation method and application thereof. The naturally screened Mortierella alpina is mutagenized to obtain a Mortierella alpina mutant strain 200012201-12-2-2, CCTCC NO:M2013392 capable of producing high-yield arachidonic acid. After the strain is subjected to long-term transformation, the hereditary characters are stable and are not mutated, the biological characteristics are typical, and the growth traits are good in a microscopy and fermentation tank, and the strain has the advantages of high C and N utilization ratio, high biomass, high oil production level, high ARA yield and good quality, and can easily extract grease and required fatty acids; and by using the fermentation method, the yield of arachidonic acid is 15.1g / L fermentation liquid.

The invention relates to a fermentation medium for promoting mortierella alpina to produce EPA (eicosapentaenoic acid). The fermentation medium for promoting the mortierella alpina to produce the EPA consists of 50g / L of starch, 30-70g / L of soybean meal extract, 0-10g / L of potassium nitrate, 1g / L of phosphate, 0.25g / L of magnesium sulfate heptahydrate and the balance of water, and PH (potential of hydrogen) value of the fermentation medium is 6.0-8.0. The fermentation medium for promoting the mortierella alpina to produce the EPA achieves the purpose of substantially improving EPA yield of the mortierella alpina CCFM695 under normal temperature environment, enables the EPA yield to reach 1.7g / L, and increases the yield by about 3 times of that under original conditions.

The invention relates to a method for producing ARA grease rich in chrome. Glucose and yeast powder rich in chrome are taken as the raw materials, the method comprises steps of fermenting mortierella alpina so as to obtain the ARA fermentation liquid rich in chrome, filtering and separating so as to obtain ARA wet thallus rich in chrome, drying then extracting through organic solvent so as to obtain crude oil, hydrating, alkali refining, decoloring and deodorizing the obtained crude oil so as to obtain the ARA grease rich in chrome. The nutrient grease can be used for lowering blood fat and preventing diabetic complication, and is good news for diabetics.

The invention relates to the technical field of microorganisms, and discloses microbial oil rich in Sn-2-site ARA as well as a preparation method and application of the microbial oil. The proportion of ARA at the Sn-2 site of triglyceride in the microbial oil is not lower than 23%. The preparation method of the microbial oil comprises the step of inoculating a fermentation medium with a mortierella alpina strain for fermentation to obtain the microbial oil, wherein the mortierella alpina strain has preservation number of GDMCC No.60734. The proportion of the ARA at the Sn-2 site of the triglyceride in the microbial oil is remarkably higher than that of the ARA at the Sn-1 site and the Sn-3 site of the triglyceride, so that the human body absorption rate of the ARA in the microbial oil is remarkably higher than that of the ARA produced by a conventional strain, and absorption and utilization of the functional fatty acid ARA by a human body are promoted.

The present invention relates to a growth regulator and a fermentation method for Mortierella alpina, one or more growth regulators of the Mortierella alpine are added into a fermentation medium to promote rapid growth of Mortierella alpina thalluses. Under the condition of a same inoculum size, the fermentation delay period is significantly shortened, the rapid growth of the Mortierella alpina thalluses can be promoted, the production efficiency of ARA oil is improved, and the production cost is reduced. The method is simple in operation, has a remarkable promoting effect on the growth of theMortierella alpina thalluses, and is suitable for industrial application of the ARA oil.

The invention relates to a method for extracting grease contained in mortierella alpine. The method comprises the following steps: (1), inoculating mortierella alpine strains into a liquid culture medium for fermentation to obtain fermentation liquor; (2), performing filtering to obtain mortierella alpine wet cells, dissolving the mortierella alpine wet cells in a sodium chloride solution to obtain a mortierella alpine cell solution, then performing emulsifying and shearing, and adjusting the temperature to 40-60 DEG C; (3), adjusting the pH value of the mortierella alpine cell solution to 6.5-7.5, adding an autolysis inducer, continuously performing stirring, and autolyzing mortierella alpine cells for 8-12 hours in a vacuum oxygen-free environment to obtain a mortierella alpine autolysis solution; (4), carrying out centrifugal separation, and collecting upper-layer grease; (5), collecting lower-layer solid obtained by centrifugal separation in the step (4), uniformly mixing the lower-layer solid with purified water, performing centrifugal separation, and collecting upper-layer grease; and (6), mixing the upper-layer grease collected in the steps (4) and (5). The method can effectively extract the grease in mortierella alpine cells, has the advantages of simple technique and low energy consumption, and is beneficial to implementing large-scale production.

The invention relates to a method for producing arachidonic acid oil (AA oil) low in nervonic acid and eicosapentaenoic acid (EPA). The method provided by the invention adopts the mortierella isabelina as the original strain, which undergoes strain activation, preparation of the strain in a shake bottle, and fermentation, then the bacteria are harvested, and the AA oil low in nervonic acid and eicosapentaenoic acid is extracted from the bacterium. With the eicosapentaenoic acid as the original strain for fermentation, the capability of oil production can be improved for 1-3 times, the yield of AA can be raised for 1-3 times; the invention has the simple fermentation condition with controllable process, and capability to produce the mixed oil of AA with high quality which is low in nervonic acid and EPA.

The present invention relates to a method for producing a phospholipid containing a long-chain polyunsaturated fatty acid as a constituent (LCPUFA-PL), which comprises: (i) culturing a microorganism belonging to Mortierella sp. under conditions allowing intracellular accumulation of LCPUFA-PL in the microorganism; and (ii) obtaining LCPUFA-PL from the cultured product of the microorganism (wherein the compositional ratio of isopalmitic acid, a branched fatty acid, is 5% or less based on the total amount of fatty acids contained as constituents of all phospholipids (PL) contained in the cultured product). The present invention also relates to the phospholipid obtained by the method of the present invention.

The arachidonic acid oil and its microbial fermentation production method relate to the microbial fermentation production method, overcome the shortcomings of low arachidonic acid content and high cost of products, and enhance production stability and food safety. The arachidonic acid oil is produced by Mortierella alpina M0223, the content of arachidonic acid residues is 65.5-73.5%, and the content of eicosapentaenoic acid residues is 0.69-3.7% of the content of arachidonic acid residues. %. The steps of the method are as follows: culturing and activating strains, culturing seeds, inserting strains into shake flasks or fermentation tanks for fermentation and culturing, collecting wet cells, drying and extracting oil. The invention greatly improves the product quality, reduces the cost, and avoids the problems of production stability and food safety caused by artificially modified strains. The refined oil of the present invention can be used as nutrition enhancer for infants and young children, food additive and raw material of medicine and cosmetics.

The invention discloses a preparation method for mortierella alpina protoplast. An enzymatic hydrolysate consists of active enzyme, an osmotic pressure stabilizing agent and water, wherein the active enzyme comprises the following components according to mixing ratio: 300-375 U / ml cellulase, 15-20 mg / ml helicase and 2500-5000 U / ml lysozyme. The preparation method comprises the following steps: inoculating activated mortierella alpina into a liquid medium, shaking culturing, collecting mycelium, and washing to be clean; adding the sterile enzymatic hydrolysate into mycelium, conducting full enzymolysis, removing mycelium scraps, collecting, and washing, so as to obtain the protoplast. The enzymatic hydrolysate can efficiently conduct enzymolysis on the cell wall of the mycelium of the mortierella alpina, so that the high-quality mortierella alpina protoplast can be prepared, and the yield of the protoplast can reach 5.5*10<7> per millilitre.

The invention relates to the technical field of extracting ARA oil from a Mortierella alpine fermentation liquor, and concretely relates to a method for extracting arochidonic acid oil in a non-solvent manner. The method which can improve the wall breaking effect on Mortierella alpine, and is simple in process, feasible in industry, capable of easily achieving large-scale production, high in oil yield, free of solvent residues, green and efficient. The method comprises the following steps: S1, separating cells; S2, carrying out air cavitation wall breaking; S3, mixing materials; S4, puffing and granulating; S5, adding water, and dispersing; S6, carrying out secondary wall breaking treatment; S7, separating ARA crude oil; and S8, separating and drying. A plurality of wall breaking modes arecombined to carry out sufficient wall breaking on Mortierella alpine cells, and no organic solvent is introduced, so the problem of solvent residues in an existing product is solved, some non-polar cell metabolites can be prevented from being brought into crude arochidonic acid oil through non-solvent extraction, the quality of the crude arochidonic acid oil is improved, and the refining pressureis reduced.

The invention discloses a CRISPR system and an application thereof to mortierella alpina, and belongs to the technical field of bioengineering. Nuclease adopted in the invention is LbCpf1 of Lachnospiraceae bacterium, an inducible promoter used in the invention is cbh1 in Trichoderma reesei, a selected targeting gene is uracil gene ura5, and a starting strain used in the invention is a mortierellaalpina uracil auxotrophic type MAU1. According to the mortierella alpina system based on a CRISPR technology, provided by the invention, no significant difference between biomass and fat production content and wild fungi can be realized, polygene expression can be achieved, and the method can provide a technical means for constructing recombinant mortierella alpina with high industrial value.

The invention discloses a cane sugar non-fermentation type protein kinase regulatory subunit and an application thereof, and belongs to the technical fields of genetic engineering and microorganism engineering. The cane sugar non-fermentation type protein kinase regulatory subunit of which the amino acid sequence is shown as SEQID No.1 has the function of responding extracellular nutrient level and adjusting and controlling lipid metabolism, and participates in response of thalli to extracellular glucose level. The gene has the negative regulation functions on growth and lipid accumulation, and can supply energy for the thalli by restraining growth of mountain mortierella and strengthening catabolism of fatty acids under the action of limitation by glucose.

The invention relates to mortierella alpina and application thereof. The mortierella alpina strain is preserved in a collection unit specified by the National Intellectual Property Administration on February 28th, 2019, the name of the collection unit is the China General Microbiological Culture Collection Center (CGMCC), and the collection number is CGMCC No.17077. The mixed mortierella agent hasa better application effect than a single mortierella alpina or mortierella elongate agent, can directly promote straw degradation and increase quick-acting carbon-nitrogen nutrients of oil and enzyme activity related to carbon, nitrogen and phosphorus, can also secrete auxin, zeatin and abscisic acid, and has the potential of promoting crop growth. The two strains of mortierella are obtained byculturing and separating from fertile farmland soil in which organic fertilizer is applied for a long time, can automatically propagate and grow in the soil environment, and are harmless to the environment, the subculture of the strains and the preparation method of the agent are simple, the cost of raw materials of the agent is low, and the agent can be popularized and applied in the agriculturalfield.

The invention provides a method for producing arachidonic acid by fermentation of mortierella alpine, which uses rapeseed meal or rapeseed meal hydrolysate as a main component of a fermentation mediumto ferment and culture the mortierella alpine. According to the fermentation method, the production cost of arachidonic acid is reduced, and the yield of arachidonic acid is increased.