Method for extracting grease contained in mortierella alpine
A technology for Mortierella alpine and oil, which is applied in the directions of microorganism-based methods, oil/fat production, biochemical equipment and methods, etc., can solve problems such as unfavorable large-scale production, reduction of oil extraction yield, instability risk, etc., To achieve the effect of reducing the risk of saponification, reducing the risk of residual, and realizing large-scale production safety
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Embodiment 1
[0034] In the present embodiment, the method for extracting the oil contained in Mortierella alpina comprises the following steps:
[0035](1) Inoculate the oil-producing microorganism Mortierella alpina into a liquid medium for fermentation to obtain a microbial fermentation liquid, specifically: use Mortierella alpina ampoule tanks to inoculate the inclined plane, carry out liquid shake flask culture, and then transfer to After the primary seed tank is cultivated, it is then transferred to a volume of 50m 3 The fermentation tank culture of wherein said liquid culture medium is made up of glucose 40g / L, yeast powder 25g / L, sodium glutamate 6g / L, the rest is water, and the pH value of adjustment medium is 6~8 (fermentation process Monitor the pH value throughout the process, use phosphoric acid and ammonia water to adjust the pH value within the range of 6~8), maintain the temperature of the fermenter at 29.5~30°C for 0~48h, and maintain the temperature of the fermenter at 27....
Embodiment 2
[0045] In the present embodiment, the method for extracting the oil contained in Mortierella alpina comprises the following steps:
[0046] (1) Inoculate the oil-producing microorganism Mortierella alpina into a liquid medium for fermentation to obtain a microbial fermentation liquid (same as Example 1);
[0047] (2) Take 1 kg of Mortierella alpina wet cells obtained by plate and frame filtration in step (2) of Example 1, and dissolve them in 1280 mL of sodium chloride solution with a concentration of 5% by weight to obtain a Mortierella alpina cell solution (Mertierella alpina cell solution) In the Mortierella alpina cell solution, the weight percent concentration of the Mortierella alpina cell is about 14% in terms of dry matter); then the Mortierella alpina cell solution is emulsified and sheared (using a high-shear emulsifier, at a rotor speed of 8000rpm In case of emulsification and shearing of the Mortierella alpina cell solution for 15 minutes), adjust the temperature o...
Embodiment 3
[0055] In the present embodiment, the method for extracting the oil contained in Mortierella alpina comprises the following steps:
[0056] (1) Inoculate the oil-producing microorganism Mortierella alpina into a liquid medium for fermentation to obtain a microbial fermentation liquid (same as Example 1);
[0057] (2) Take 1 kg of Mortierella alpina wet cells obtained by plate and frame filtration in step (2) of Example 1, and dissolve them in 1280 mL of sodium chloride solution with a concentration of 5% by weight to obtain a Mortierella alpina cell solution (Mertierella alpina cell solution) In the Mortierella alpina cell solution, the weight percent concentration of the Mortierella alpina cell is about 14% in terms of dry matter); then the Mortierella alpina cell solution is emulsified and sheared (using a high-shear emulsifier, at a rotor speed of 12000rpm In case of emulsification and shearing of the Mortierella alpina cell solution for 6 minutes), adjust the temperature o...
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