Method for promoting production of mortierella alpina and oil yield of arachidonic acid

Abstract

The invention relates to a method for promoting production of mortierella alpina and oil yield of arachidonic acid. The method comprises the following steps that fermented clear liquid of the mortierella alpina is collected, and through extraction and concentration of hydrophilic organic solvent, an accelerating agent which can adjust and control the production of the mortierella alpine and the oil yield of the arachidonic aicd is obtained by screening; and in the 0-48 hours at the early stage of fermentation, the accelerating agent is added to a normal medium according to the ratio of 0.1-0.5%, and a conditioned medium is prepared to inoculate and cultured at the temperature of 28 DEG C. After the fermentation, in fermentation results of some embodiments, biomass is 43.2 g / L, oil content is 61.3%, ARA content is 60.7%, ARA yield is 16.1 g / L, and the fermentation period is shortened from 192 hours to 168 hours. The method for promoting the production of the mortierella alpina and the oil yield of the arachidonic acid is simple and effective, promotes the production of the mortierella alpine, improves the oil yield of the arachidonic acid, and is of great significance to industrial application.

Description

technical field [0001] The invention relates to the field of microbial fermentation, in particular to a method for promoting the growth of Mortierella alpina and the yield of arachidonic acid oil. Background technique [0002] Arachidonic acid (ARA) is a long-chain polyunsaturated fatty acid with very important physiological functions. It is an important nutrient that cannot be synthesized by the human body itself but is indispensable. ARA exists in large quantities in the nerves of the human retina and cerebral cortex. In the tissue, it is the basis of the brain development of fetuses and infants, and it has a promoting effect on their intelligence and cognitive ability. [0003] Improving ARA production by optimizing fermentation conditions is the focus of current research. Chinese patent 201310475443.1 discloses a method for increasing ARA production through mutagenesis and medium optimization. Although ARA production has been greatly improved, the mutagenesis is random a...

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Problems solved by technology

[0003] Improving ARA production by optimizing fermentation conditions is the focus of current research. Chinese patent 201310475443.1 discloses a method for increasing ARA production through mutagenesis and medium optimization. Although ARA production has been greatly improved, the mutagenesis is random and The mutated strain is genetically unstable, easily leads to back mutation, and is not easy for industrial application

Chinese patent CN201410556081.3 discloses a method for producing arachidonic acid oil based on the regulation of Mortierella alpina fermentation by dissolved oxygen. This method mainly increases the yield of ARA by regulating the high concentration of dissolved oxygen, but the yield of ARA also increases Reduced energy consumption and increased production costs

[0004] At present, although the production of ARA oil by fermentation of the microorganism Mortierella alpina has been industrialized, there are still problems such as long production cycle and low production efficiency in the industrial production process.

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