Mortierella elongata PFY capable of utilizing lignocellulose for fermentation production of microbial oil and method of producing microbial oil by same
A technology of microbial oil and lignocellulose, which is applied in the directions of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of high production cost, low production efficiency, and cellulase increase production cost, and achieve the fermentation cycle. Short, efficient and rapid utilization, easy to cultivate and grow effect
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Examples
Embodiment 1
[0022] Example 1: After gradient dilution of the soil samples, 0.2 mL was evenly spread on the Congo red medium, cultured at 30°C, and the colony growth on the medium was observed every day, and the size of the colony and the diameter of the formed transparent circle were recorded. The fast-growing colonies were picked and re-inoculated on Congo red medium by the three-part streak method for isolation and purification to obtain single colonies, and the fast-growing single colonies were selected as the target microorganisms for preservation. Among them, the formula of Congo red medium is (g / L): K 2 HPO 4 0.5-1.0, MgSO 4 0.2-0.5, microcrystalline cellulose powder 1.0-2.0, Congo red 0.1-0.5, agar 10-20, gelatin 1-2, pH natural.
Embodiment 2
[0023] Example 2: Observation of microorganisms found that the mycelia of the strain were white at first, and then turned gray-green, the colony was limited, and the mycelia grew prostrate; the mycelia had septa, branches, and no rhizoids; the sporangia were upright, and the sporangia were rod-shaped or oval Spherical; hyphae transparent or light green, spores clustered, spores light green. The results of ITS full sequence analysis showed that the strain was Mortierella longosporum ( Mortierella elongata ) sp. Mortierella longosporum is a fungus of the genus Mortierella; it is classified as Mortierella longospora, and it has been preserved by the General Microorganism Center of the China Microbiological Culture Collection Management Committee, and the registration number of the preservation center is 5070.
Embodiment 3
[0024] Example 3: Fermentation was carried out with rice straw that had not been pretreated as a substrate, and the screened strain Mortierella longosporum PFY was inoculated in the fermentation medium, and cultured at 30°C for 5 days. After the fermentation was completed, the materials in the shake flask were filtered and baked. Dry, the quality after drying is about 70% of the feed amount, and the oil content in the dry matter is about 4%.
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com