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Mortierella elongata PFY capable of utilizing lignocellulose for fermentation production of microbial oil and method of producing microbial oil by same

A technology of microbial oil and lignocellulose, which is applied in the directions of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of high production cost, low production efficiency, and cellulase increase production cost, and achieve the fermentation cycle. Short, efficient and rapid utilization, easy to cultivate and grow effect

Inactive Publication Date: 2012-08-01
安徽安生生物化工科技有限责任公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Usually, there are problems such as low production efficiency and high production cost when microbial fermentation is used to process and utilize these waste materials, and it is necessary to combine other physical or chemical methods to pretreat the materials, and sometimes multi-step enzymatic hydrolysis treatment and fermentation are required to obtain products.
Use enzymatic saccharification liquid for fermentation production, although microorganisms can easily use the saccharification liquid produced by enzymatic hydrolysis for fermentation, but this requires the addition of cellulase and thus increases production costs
There are many reports on cellulosic ethanol as the product produced by fermentation of lignocellulose, but there are few reports on the production of oil by fermentation, and there are fewer studies on the direct fermentation of lignocellulose by a single strain to produce biological products

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1: After gradient dilution of the soil samples, 0.2 mL was evenly spread on the Congo red medium, cultured at 30°C, and the colony growth on the medium was observed every day, and the size of the colony and the diameter of the formed transparent circle were recorded. The fast-growing colonies were picked and re-inoculated on Congo red medium by the three-part streak method for isolation and purification to obtain single colonies, and the fast-growing single colonies were selected as the target microorganisms for preservation. Among them, the formula of Congo red medium is (g / L): K 2 HPO 4 0.5-1.0, MgSO 4 0.2-0.5, microcrystalline cellulose powder 1.0-2.0, Congo red 0.1-0.5, agar 10-20, gelatin 1-2, pH natural.

Embodiment 2

[0023] Example 2: Observation of microorganisms found that the mycelia of the strain were white at first, and then turned gray-green, the colony was limited, and the mycelia grew prostrate; the mycelia had septa, branches, and no rhizoids; the sporangia were upright, and the sporangia were rod-shaped or oval Spherical; hyphae transparent or light green, spores clustered, spores light green. The results of ITS full sequence analysis showed that the strain was Mortierella longosporum ( Mortierella elongata ) sp. Mortierella longosporum is a fungus of the genus Mortierella; it is classified as Mortierella longospora, and it has been preserved by the General Microorganism Center of the China Microbiological Culture Collection Management Committee, and the registration number of the preservation center is 5070.

Embodiment 3

[0024] Example 3: Fermentation was carried out with rice straw that had not been pretreated as a substrate, and the screened strain Mortierella longosporum PFY was inoculated in the fermentation medium, and cultured at 30°C for 5 days. After the fermentation was completed, the materials in the shake flask were filtered and baked. Dry, the quality after drying is about 70% of the feed amount, and the oil content in the dry matter is about 4%.

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Abstract

The invention discloses a Mortierella elongata PFY capable of utilizing lignocellulose for fermentation production of microbial oil and a method of producing microbial oil by the same. The method mainly comprises the following steps: culturing and screening strains by using a Congo red culture medium; inoculating the strains into a medium containing lignocellulose for culturing; drying and crushing fermented substances; and extracting crude oil in the dried substances through extraction in an organic solvent. According to ITS complete sequence analysis, the screened strains are Mortierella fungi; the strains grow rapidly and can highly efficiently utilize lignocellulose, the utilization rate of lignocellulose is as high as 90% after fermentation is finished, and the dried substances obtained after fermentation contain a great number of microbial oil, wherein the content of unsaturated fatty acid is 59%.

Description

technical field [0001] The invention relates to a new bacterial strain for fermenting and producing unsaturated fatty acid by using pretreated lignocellulose as a substrate. Background technique [0002] Usually, there are problems such as low production efficiency and high production cost when microbial fermentation is used to process and utilize these waste materials, and it is necessary to combine other physical or chemical methods to pretreat the materials, and sometimes multi-step enzymatic hydrolysis treatment and fermentation are required to obtain products. . Enzymatic hydrolysis and saccharification liquid are used for fermentation production. Although microorganisms can easily use the saccharification liquid produced by enzymatic hydrolysis for fermentation, this requires the addition of cellulase and thus increases production costs. There are many reports on the production of cellulosic ethanol by fermentation of lignocellulose, but there are few reports on the p...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12P7/64C12R1/645
Inventor 姚日生张鹏王淮邓胜松张遥姚力蓉
Owner 安徽安生生物化工科技有限责任公司
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