Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for producing ARA grease rich in chrome

A production method and oil technology, applied in the field of chromium-rich ARA oil production, can solve problems such as poor blood sugar control, and achieve the effect of preventing diabetic complications

Inactive Publication Date: 2014-03-26
WUHAN SHUTAI TECH
View PDF2 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Patients with type II diabetes produce a large amount of insulin, but their blood sugar is not well controlled. With the supplement of chromium, endogenous insulin decreases and glucose tolerance improves

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] A method for producing chromium-rich ARA grease, the steps are as follows:

[0030] ⑴ Bacteria activation culture

[0031] The slant medium formula is as follows:

[0032] 30g potatoes, 2g glucose, 0.25g chromium-enriched yeast powder, 1.5g agar, 100ml water;

[0033] The activation of Mortierella alpina strains was cultured on a slant, with a culture temperature of 28°C and a culture time of 7 days;

[0034] ⑵ shake flask culture

[0035] The volume concentration of the shake flask medium formula is as follows:

[0036] Glucose 20g / L, chromium-enriched yeast powder 2.5g / L;

[0037] Use slant strains for liquid shake flask culture, the culture temperature is 28°C, the rotation speed is 140rpm, and the culture time is 7 days;

[0038] ⑶ fermentation culture

[0039] The volumetric concentration of the fermentation medium is as follows:

[0040] Glucose 30g / L, chromium-enriched yeast powder 20g / L;

[0041] ①First-level fermentation culture: transfer the shake flas...

Embodiment 2

[0048] A method for producing chromium-rich ARA grease, the steps are as follows:

[0049] ⑴ Bacteria activation culture

[0050] The slant medium formula is as follows:

[0051] 30g potato, 4g glucose, 0.5g chromium-enriched yeast powder, 2g agar, 100ml water;

[0052] The activation of Mortierella alpina strains was cultured on a slope with a culture temperature of 30°C and a culture time of 10 days;

[0053] ⑵ shake flask culture

[0054] The volume concentration of the shake flask medium formula is as follows:

[0055] Glucose 40g / L, chromium-enriched yeast powder 5g / L;

[0056] Use slant strains for liquid shake flask culture, culture temperature 30°C, rotation speed 200rpm, culture time 10 days;

[0057] ⑶ fermentation culture

[0058] The volumetric concentration of the fermentation medium is as follows:

[0059] Glucose 60g / L, chromium-enriched yeast powder 30g / L;

[0060] ①First-level fermentation culture: transfer the shake flask strain in step ⑵ into a ferme...

Embodiment 3

[0067] A method for producing chromium-rich ARA grease, the steps are as follows:

[0068] ⑴ Bacteria activation culture

[0069] The slant medium formula is as follows:

[0070] Potato 30g, glucose 3g, chromium-enriched yeast powder 0.38g, agar 1.75g, water 100ml;

[0071] Mortierella alpina strains were activated by cultured on a slant, with a culture temperature of 29°C and a culture time of 8 days;

[0072] ⑵ shake flask culture

[0073] The volume concentration of the shake flask medium formula is as follows:

[0074] Glucose 30g / L, chromium-enriched yeast powder 3.8g / L;

[0075] The slant strain was used for liquid shake flask culture, the culture temperature was 29°C, the rotation speed was 170rpm, and the culture time was 9 days;

[0076] ⑶ fermentation culture

[0077] The volumetric concentration of the fermentation medium is as follows:

[0078] Glucose 45g / L, chromium-enriched yeast powder 25g / L;

[0079] ①First-level fermentation culture: transfer the shak...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a method for producing ARA grease rich in chrome. Glucose and yeast powder rich in chrome are taken as the raw materials, the method comprises steps of fermenting mortierella alpina so as to obtain the ARA fermentation liquid rich in chrome, filtering and separating so as to obtain ARA wet thallus rich in chrome, drying then extracting through organic solvent so as to obtain crude oil, hydrating, alkali refining, decoloring and deodorizing the obtained crude oil so as to obtain the ARA grease rich in chrome. The nutrient grease can be used for lowering blood fat and preventing diabetic complication, and is good news for diabetics.

Description

technical field [0001] The invention relates to a production method of arachidonic acid (ARA) oil. Specifically, it relates to a method for producing arachidonic acid (ARA for short) oil by fermenting chromium-rich yeast powder as a raw material. Background technique [0002] "Chromium" is an essential trace element for the human body. A normal human body only contains 6-7 mg, but it is very important and sensitive to the human body. As long as 1 microgram of chromium is given per kilogram of body weight, it is enough to show its biological function. Chromium is widely distributed in various organs, tissues and body fluids in the body. The main function of chromium is to play a role in glucose metabolism. Organic "chromium" element and insulin Human body's tolerance to glucose is regulated by glucose tolerance factor, and chromium element is an indispensable factor for the stability of glucose tolerance factor. Glucose tolerance factor can promote the action of insulin...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12P7/64C12R1/645
Inventor 简晓红
Owner WUHAN SHUTAI TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com