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Growth regulator and fermentation method of Mortierella alpina

A growth regulator, the technology of Mortierella alpina, which is applied in the field of growth regulator and fermentation to promote the rapid growth of Mortierella alpina, can solve the problems of low production efficiency, slow bacterial production, low ARA output, etc., to improve production Efficiency, promoting rapid growth, shortening the effect of fermentation delay period

Active Publication Date: 2018-07-24
HUAZHONG UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved by the present invention is to provide a growth regulator and fermentation method of Mortierella alpina, so as to solve the problems of low production efficiency of Mortierella alpina fermentation, slow cell production, and low ARA yield

Method used

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  • Growth regulator and fermentation method of Mortierella alpina
  • Growth regulator and fermentation method of Mortierella alpina
  • Growth regulator and fermentation method of Mortierella alpina

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] (1) Preparation of Mortierella alpina seed solution

[0028] Inoculate Mortierella alpina, a slant strain preserved in a refrigerator at 4°C, into PDA medium for 7 days of activation to prepare spores, add 50 mL of sterile saline with glass beads to prepare spore liquid, and inoculate the spore liquid at an inoculum volume of 8%. Insert the seed medium into the culture medium at 28°C for 48 hours to prepare the seed solution;

[0029] Seed medium composition (g / L): glucose 50.0g / L, yeast powder 10.0g / L, sodium nitrate 3.0g / L, potassium dihydrogen phosphate 3.0g / L, magnesium sulfate heptahydrate 0.5g / L.

[0030] (2) The seed solution is inserted into the fermentation medium at 28°C according to the inoculum amount of 2% for cultivation, and different Mortierella alpina growth regulators are added respectively;

[0031] Group A: p-Hydroxyphenethylamine 20μmol / L

[0032] Group B: p-hydroxyphenylacetic acid 20μmol / L

[0033] Group C: p-hydroxyphenylpropionic acid 20μmol / ...

Embodiment 2

[0049] (1) Preparation of Mortierella alpina seed solution is the same as in Example 1.

[0050] (2) The seed solution is inserted into the fermentation medium according to 10% inoculum amount for cultivation at 28° C., and different Mortierella alpina growth regulators are added respectively;

[0051]Group A: p-Hydroxyphenethylamine 100μmol / L

[0052] Group B: p-hydroxyphenylacetic acid 100μmol / L

[0053] Group C: p-hydroxyphenylpropionic acid 100μmol / L

[0054] Group D: phenethylamine 100 μmol / L

[0055] Group E: 2-phenylethanol 100μmol / L

[0056] Group F: p-hydroxyphenethyl alcohol 100 μmol / L

[0057] Group G: p-hydroxyphenylethylamine 20 μmol / L, p-hydroxyphenylacetic acid 40 μmol, p-hydroxyphenylpropionic acid 40 μmol / L

[0058] Group H: phenethylamine 50 μmol / L, 2-phenylethyl alcohol 20 μmol / L, p-hydroxyphenethyl alcohol 30 μmol / L

[0059] CK1: DMSO

[0060] CK2: do not add

[0061] Composition and concentration of fermentation medium (g / L): glucose 100.0, yeast p...

Embodiment 3

[0067] (1) Preparation of Mortierella alpina seed solution is the same as in Example 1.

[0068] (2) The seed solution is inserted into the fermentation medium at 28°C according to an 8% inoculum amount for cultivation, and different Mortierella alpina growth regulators are added respectively;

[0069] Group A: p-Hydroxyphenethylamine 80μmol / L

[0070] Group B: p-hydroxyphenylacetic acid 80μmol / L

[0071] Group C: p-hydroxyphenylpropionic acid 80μmol / L

[0072] Group D: phenylethylamine 80μmol / L

[0073] Group E: 2-phenylethanol 80μmol / L

[0074] Group F: 80 μmol / L p-hydroxyphenethyl alcohol

[0075] Group G: p-hydroxyphenylethylamine 20 μmol / L, p-hydroxyphenylethyl alcohol 60 μmol / L

[0076] Group H: p-hydroxyphenylacetic acid 40 μmol / L, phenethylamine 40 μmol / L

[0077] Group I: 2-phenylethyl alcohol 20 μmol / L, p-hydroxyphenylpropionic acid 60 μmol / L

[0078] CK1: DMSO

[0079] CK2: do not add

[0080] Fermentation medium composition and concentration (g / L): glucose...

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Abstract

The present invention relates to a growth regulator and a fermentation method for Mortierella alpina, one or more growth regulators of the Mortierella alpine are added into a fermentation medium to promote rapid growth of Mortierella alpina thalluses. Under the condition of a same inoculum size, the fermentation delay period is significantly shortened, the rapid growth of the Mortierella alpina thalluses can be promoted, the production efficiency of ARA oil is improved, and the production cost is reduced. The method is simple in operation, has a remarkable promoting effect on the growth of theMortierella alpina thalluses, and is suitable for industrial application of the ARA oil.

Description

technical field [0001] The invention relates to the field of microbial fermentation, in particular to a growth regulator and a fermentation method for promoting the rapid growth of Mortierella alpina. Background technique [0002] Arachidonic acid (ARA) is a long-chain polyunsaturated fatty acid with very important physiological functions. It is an important nutrient that cannot be synthesized by the human body itself but is indispensable. ARA exists in large quantities in the nerves of the human retina and cerebral cortex. In the tissue, it is the basis of fetal and infant brain development, and it can promote its intelligence and cognitive ability, and ARA is a variety of eicosanoid derivatives such as prostaglandin E2 (PGE2), prostacyclin (PGI2), thrombo The direct precursor of physiologically active substances such as alkane A2 (TXA2) and leukotriene and C4 (LTC4), these biologically active substances have effects on lipid protein metabolism, blood rheology, blood vessel...

Claims

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Application Information

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IPC IPC(8): C12N1/38C12N1/14C12P7/64C12R1/645
CPCC12N1/14C12N1/38C12P7/6427
Inventor 余龙江朱圆敏朱师博
Owner HUAZHONG UNIV OF SCI & TECH
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